Neuroprotective effects of orientin in rats after cerebral ischemia-reperfusion
10.13699/j.cnki.1001-6821.2018.05.022
- VernacularTitle:荭草苷对脑缺血再灌大鼠的神经保护作用
- Author:
Xiao-Ru WANG
1
;
Yun-Guang DU
;
Juan YAN
;
Shu-Hua WANG
;
Fang AN
Author Information
1. 河北北方学院
- Keywords:
orientin;
cerebral ischemia-reperfusion;
autophagy;
neuroprotective
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(5):565-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the neuroprotective effects of orientin in rats after cerebral ischemia-reperfusion and its possible mechanisms.Methods SD male rats were randomly divided into five groups,with 15 rats in each group:sham group,model group,control group,experimental Ⅰ group,experimental Ⅱ group.Middle cerebral artery occlusion (MCAO) model was established by suture method in each group except sham group.For sham group,separate the carotid artery only.The 2.9 mg · mL-1 of orientin was administrated at 0 h and 12 h of reperfusion in control group and two experimental groups.At 0.5,24 h before the operation,autophagy inducer (rapamycin 2.24 mg · mL-1) and autophagy inhibitor [3-methyladenine (3-MA) 3.0 mg · mL-1] were administrated in experimental Ⅰ group and experimental Ⅱ group,respectively.The same dosage of normal saline was administrated to other groups.The neurological deficit scores were evaluated and brain infarct volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining after 24 h of reperfusion.Moreover,protein immunoblotting was used to observe the protein levels of Beclin1 and microtubule-associated protein1 light chain3 (LC3).Results The brain infarct volume in model group was (36.63 ±2.06)％,which was higher than sham group (0.67 ±0.12)％,the difference was statistically significant (P ＜0.01).The brain infarct volume in control group,experimental Ⅰ and Ⅱ group were (14.71 ± 1.63)％,(25.22 ± 1.58) ％ and (6.45 ± 1.07) ％,respectively;compared with model group and three drugs groups,the difference were statistically significant (all P ＜ 0.05).The expression of autophagy protein Beclin1 (APB) in model group,sham group were 3.16 ±0.17,(1.00 ±0.06) while control group,experimental Ⅰ and Ⅱ groups were,1.67 ±0.15,2.24 ±0.13 and 1.21 ±0.09,respectively.For the protein expression of LC3 in model group,sham group,control group,experimental Ⅰ and Ⅱ groups were 2.98 ± 0.12,1.00 ± 0.05,1.54 ± 0.13,2.24 ± 0.12,1.49 ± 0.17.Compared with sham group and model group,the difference were statistically significant (all P ＜0.01).Compared with model group and three drugs groups,the difference were statistically significant (all P ＜ 0.05).Compared with control group and experimental Ⅰ group,the difference of the protein expression were statistically significant (all P ＜ 0.05).Conclusion The neuroprotective effects of orientin in rats after cerebral ischemia-reperfusion may be mediated through inhibition of autophagy.
