Toxicity mechanism of the unsaturated fatty acids on human cervical carcinoma of ME-180 cells
10.13699/j.cnki.1001-6821.2018.05.018
- VernacularTitle:不饱和脂肪酸对人宫颈癌细胞毒性作用机制研究
- Author:
Fa-Rong YU
1
;
Wang-Jun CHEN
;
Xiu-Zhen LIAN
;
Ming-Ren XIE
;
Deng-Lou LI
;
Yun-Xuan GUO
;
Bo YANG
Author Information
1. 甘肃政法学院 甘肃省证据科学技术研究与应用重点实验室
- Keywords:
unsaturated fatty acids;
ME-180 cells;
toxic effect;
inhibition rate;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(5):552-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cytotoxic effect mechanism of the unsaturated fatty acids on human cervical carcinoma of ME-180 cells.Methods The 2 × 108 cell · L-1 ME-180 cells were moved into the 96 hole culture plate,per hole 90 μL.Three concentrations of the drug groups:0.3,0.6,0.9 mg· L-1 unsaturated fatty acid (prepared with DMSO) per hole 90 μL.The 5-fluorouracil(5-FU) group:10mg · L-1 5-FU (prepared with phosphate buffer) per hole 10 μL.Control group:30 μL · L-1 DMSO per hole 10 μL.Each group set of four repeat hole,in saturated humidity condition of 37 ℃ and 5％ CO2 in 48 h.The inhibition rate and apoptosis rate of unsaturated fatty acids were detected with a trypan blue,thiazole blue (MTT),and flow cytometry method.Results When ME-180 cells were administered with the unsaturated fatty acids at the doses of 0.3,0.6,0.9 mg · L-1 for 48 h,ME -180 living cell count with the increase of drug concentration and action time is reduced.The cells proliferation inhibition rate were 43.35％,51.75％,66.45％ in three concentrations of the drug groups;apoptosis rate were 18.21％,23.11％,28.06％ in the three groups,respectively.Compared with the drug groups and the control group on the number of living cells,inhibition rate and apoptosis rate,the differences were statistically significant (P ＜0.01).Conclusion The unsaturated fatty acids has significant toxic effects on ME-180 cells.The toxic effect mechanism of the unsaturated fatty acids is related to inhibite proliferation on cell and induce-cell apoptosis.
