Spectrum and toxicity relationship of different extracts of Aconitum Sinomontanum Nakai on acute toxicity
10.13699/j.cnki.1001-6821.2018.04.022
- VernacularTitle:高乌头不同溶剂萃取部分急性毒性的谱-毒关系研究
- Author:
Li-Jun ZHANG
1
;
Yun LI
;
Hai-Rong DAI
;
Zhi-Jun YANG
;
Qin FAN
;
Jun MA
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Aconitum Sinomontanum Nakai;
different extract;
fingerprint;
acute toxicity;
spectrum and toxicity relationship
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(4):470-474
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the contribution of the fingerprint peaks from different extracts of Aconitum Sinomontanum Nakai (ASN) to the acute toxicity,and to reflect the material basis.Methods The experimental drugs (petroleum ether,chloroform,ethyl acetate,butanol,alcohol and water extracts) were obtained by the means of systematic solvent extraction from the 95％ ethanol extract of ASN,The HPLC fingerprints of different extracts of ASN were established.The acute toxicity of different extracts in mice were carried out by the way of measuring the median lethal dose (LD50) and maximum dose.The relationship between spectrum and toxicity was established by gray relational analysis.Results The fingerprints of different extracts of ASN were established.The acute toxicity was not characterized only by Lappacontine (LAP),and the contribution of other diterpenoid alkaloids should not be neglected,the contribution of different peaks to toxicity was ranked as X13 ＞ X14 ＞ X12 ＞X6 ＞ X2 ＞ X9 ＞ X5 ＞ X4.Conclusion The established HPLC method for fingerprints of different extracts of ASN is simple and repeatable.The relationship between the fingerprints of different extracts and the acute toxicity is paralleled to some extent.And this will lay a foundation for the research of toxicity mechanism of ASN.
