Determination of Rosuvastatin concentration in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2018.04.021
- VernacularTitle:高效液相色谱-质谱联用法测定人血浆中瑞舒伐他汀的浓度
- Author:
Li-Jun ZHANG
1
;
Long-Mei CHENG
;
Yao-Yao LEI
;
Kun-Ming GU
;
Meng-Meng HU
;
Yuan-Yuan LI
;
Xiao-Fang WANG
;
Rui-Hua DONG
;
Heng-Yan QU
Author Information
1. 中国人民解放军第三○七医院临床药理学研究室
- Keywords:
rosuvastatin;
HPLC-MS/MS;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(4):467-469,474
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the rosuvastatin concentration in human plasma by establishing HPLC-MS/MS method.Method The plasma was treated with liquid-liquid extraction,using rosuvastatin-D6 as internal standard.Chromatographic column:Thermo(R) HypersⅡ GOLD (2.1 mm × 100 mm,3.0 μm),column temperature:40 ℃,mobile phase:Methanol-0.1％ formic acid and 5 mmol · L-1 ammonium formate,isocratic elution,flow rate:0.35 mL · min-1,mass concentration of the sample was measured with positive ion scan and multiple reaction monitoring mode.The specificity,lower limit of quantitation,standard curve,precision and accuracy,recovery and stability as well as the matrix effect were investigated.Result The standard curve of rosuvastatin was y =3.29 × 10-1 x-3.46 × 10-3 (r =0.998 8),and rosuvastatin had good linear relationship in 0.05-25.00 ng · mL-1,the lower limit of quantitation of the methodwas 0.05 ng · mL-1.Intra-day and inter-day RSD of the plasma samples were less than 15％,the average recovery was ＞ 95％,and stability was good.Conclusion The method is simple,rapid,sensitive and accurate,specific,which is suitable for the determination of rosuvastatin concentration in human plasma.
