Effects of oxymatrine on proliferation and expression of vascular endothelial growth factor in gastric cancer cell SGC-7901
10.13699/j.cnki.1001-6821.2018.04.014
- VernacularTitle:氧化苦参碱对SGC-7901胃癌细胞增殖及对血管内皮生长因子表达的影响
- Author:
Yong-Hua LIU
1
;
Mei-Zhen GUO
Author Information
1. 黄河科技学院医学院
- Keywords:
oxymatrine;
SGC-7901 gastric cancer cell;
proliferation;
vascular endothelial growth factor
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(4):443-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of oxymatrine (OM) on the proliferation and expression of vascular endothelial growth factor (VEGF) in human gastric cancer cell SGC-7901.Methods SGC-7901 cells were cultured with 0.5,1.0,2.0,4.0,8.0 g · L-1 oxymatrine (test groups),control group given no drug treatment.The proliferation of gastric cancer cell SGC-7901 after cultured 1,2,3,4 d were detected by MTr method.SGC-7901 cells were treated with 1.0,2.0 and 4.0 g · L-1 oxymatrine and cultured for 48 h.The VEGF mRNA expression of each group was detected by real-time fluorescence quantitative polymerase chain reaction (qPCR).Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of VEGF protein in each group.Results On day 4 of cell culture,the proliferation inhibition rates of control group and 0.5,1.0,2.0,4.0 and 8.0g·L-1 test groups were (4.15 ±3.93)％,(14.79 ±1.66) ％,(31.65 ±2.07) ％,(41.57 ±2.93) ％,(64.37 ±4.13) ％ and (79.17 ±4.75) ％.Compared with control group,the proliferation inhibition rate of SGC-7901 gastric cancer cells in each test group was significantly increased at different concentration (P ＜ 0.01).The proliferation inhibition rate of test group increased with the increasing of culture time and drug concentration (P ＜ 0.05or P ＜0.01).After exposuring to oxymatrine for 48 h,the expression of VEGF mRNA in control group and 1.0,2.0 and 4.0 g · L-1 test groups were 0.82 ± 0.03,0.65 ± 0.05,0.54 ± 0.06 and 0.46 ± 0.04.Compared with control group,the expression of VEGF mRNA in test group was significantly decreased,and the expression of VEGF mRNA decreased gradually with the increasing of oxymatrine concentration,the difference was statistically significant (P ＜0.01).The expression of VEGF protein in control group and 0.5,1.0,2.0,4.0,8.0 g · L-1 test groups were (1326.52 ± 139.57),(1305.69 ± 119.88),(1235.59 ± 102.36),(1083.33 ± 89.65),(789.85 ± 95.26) and (426.59 ±83.16) pg · mL-1.Compared with control group,the expression of VEGF protein in 2.0,4.0 and 8.0 g · L-1 test groups were decreased gradually with the increase of oxymatrine concentration (P ＜ 0.05).Conclusion Oxymatrine can inhibit the proliferation of SGC-7901 gastric cancer cells,and the inhibitory rate of proliferation is proportional to the action time and drug concentration,meanwhile,oxymatrine can inhibit the expression of related angiogenesis factors.
