Protective effect and the influence on related metabolite substances of Hedysarum polybotrys polysaccharides in local cerebral cerebral ischemia of rats
10.13699/j.cnki.1001-6821.2018.04.011
- VernacularTitle:红芪多糖对局灶性脑缺血大鼠脑损伤的保护作用及对相关代谢物质的影响
- Author:
Jing SHAO
1
;
Guo-Tai WU
;
Zhi-Wang WANG
;
Li-Dong DU
;
Yuan REN
;
Mei GUO
Author Information
1. 甘肃中医药大学药学院
- Keywords:
Hedysarum polybotrys polysaccharides;
local cerebral cerebral ischemia;
neurological function;
brain tissue damage
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(4):432-435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Hedysarum po-ybotrys polysaccharides (HPS) on superoxide dismutase (SOD),catalase (CAT),maleic dialdehyde (MDA),nitric oxide (NO),nitric oxide synthetase (NOS),endothelin (ET),calcitonin generelated peptide (CGRP) in local cerebral cerebral ischemia of rats.Methods The SPF degree Wister rats were randomly divided into six groups:model,sham orperation,control (Ginkgo leafdispersible tablet,0.21 g · kg-1),experimental-H,experimental-M,and experimental-L (HPS,0.60,0.3,0.15 g · kg-1) groups.Each group had ten rats.Local cerebral cerebral ischemia rat models were made by 50％ FeCl3on middle cerebral artery.Neurological score was made by blind method of level 5 and 4 points scoring method.Brain water content was detected by weight method.Brain infarction area was detected by TTC staining method.The SOD,CAT,MDA of brain tissue and NO,NOS,ET,CGRP of serum were detected by kits.Results Compared with model group and sham operation group,all factors had significant difference (all P ＜ 0.01).After taking the medicine,the SOD (U · mg-1) in model,experimental-H and experimental-M groups were 107.90 ± 30.12,135.06 ±3.21,129.32 ±20.71;the MDA(μmol · g-1)in the three groups were 6.39 ±2.03,4.08 ± 1.54,4.53 ± 1.21;the NO(μmol · L-1) in the three groups were 66.32 ± 20· 39,48.50 ±20.86,52.39 ±27.01;the NOS(U · mL-1)in the three groups were 29.95 ± 8.05,21.94 ±9.98,20.55 ± 8.06;the ET(pg · mL-1) in the three groups were 96.64 ± 21.51,75.88 ± 19.52,76.42 ±17.76;the CGRP(pg · mL-1) in the three groups were 53.98 ± 10.95,76.43 ± 22.53,67.07 ± 12.12,the differences of all above factors between groups were statistically significant (all P ＜ 0.05).Conclusion The experimental-H and experimental-M groups can protect nerve function injury and brain tissueinjury and improve the damage of the brain tissue antioxidant system in the rat model of focal cerebral ischemia.
