Effect of epigallocatechin-3-gallate on the crulein-induced acute pancreatitis associated lung injury in mice
10.13699/j.cnki.1001-6821.2018.04.009
- VernacularTitle:表没食子儿茶素没食子酸酯对雨蛙素导致的小鼠急性胰腺炎肺损伤的作用
- Author:
Hong-Fang TUO
1
;
Hui-Yan MENG
;
Wei-Zheng YANG
;
Wan-Xing ZHANG
;
Jian-Jun ZHANG
;
Yan-Hui PENG
Author Information
1. 河北省人民医院普外三科
- Keywords:
acute pancreatitis;
acute lung injury;
epigallocatechin-3-gallate;
malondialdehyde;
nuclear factor of kappa B
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(4):425-427
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of epigallocatechin-3-gallate(EGCG) on acute pancreatitis associated lung injury (APALI) in mice.Methods The balb/c mice were randomly divided into three groups:control group,model group and experimental group.APALI model mice were induced by intraperitoneal injection of crulein (50 μg · kg-1 body weight) in 0.9％ NaCl solution once per hour for 7 times.In control group,mice were only received the same volume of 0.9％ NaCl without caerulein.In experimental group,EGCG (25 mg· kg-1) was injected in abdomen at 1,3 and 6 h after the induction of APALI.Mice from each group were killed,then the correlated indexes were evaluated at 24 h after the induction of APALI.The levels of anylase,tumor necrosis factor (TNF-α),malondialdehyde (MDA) in serum and nuclear factor of kappa B (NF-кB) activity in lung tissue were determined.Hematoxylin-eosin (HE) staining was performed to evaluate the morphologic changes in animal lung.Results Levels of amylase in experimental group,model group and control group were (3613.21 ± 351.87),(4720.43 ± 672.24),(895.41 ± 107.18) U · L-1;MDA levels in the three groups were (13.06 ±0.11),(15.49 ±0.40),(4.26 ±0.69) mmol · mL-1;TNF-αt levels in the three groups were (59.83 ± 14.74),(83.29 ±24.10),(24.76 ± 10.24) pg · L-1;expression of NF-κB in the three groups were 0.38 ± 0.14,0.55 ± 0.12,0.14 ± 0.09;Histopathological scores in the three groups were (3.47 ± 1.20),(6.54 ±0.51),(0.21 ±0.07) point.Compared with control group,the differences of the factors in model group were statistically significant (all P ＜0.05).Compared with model group,the differences of the factors in experimental group were statistically significant (all P ＜ 0.05).Conclusion EGCG can ameliorate inflammation in pancreases and lungs through inhibition of NF-κB signaling pathways and improvement of oxygen free radical scavenging.
