Determination of the concentration of voriconazole in human plasma by HPLC
10.13699/j.cnki.1001-6821.2018.03.038
- VernacularTitle:高效液相色谱法测定人血浆中伏立康唑的含量
- Author:
Min LI
1
;
Chao-Yang CHEN
;
Jun-Yu XU
;
Ling-Yue MA
;
Zi-Sheng KANG
;
Xin-Ran LI
;
Xia ZHAO
;
Ying ZHOU
;
Yi-Min CUI
Author Information
1. 北京大学第一医院药剂科
- Keywords:
voriconazole;
metabolite;
HPLC;
plasma;
saliva
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(3):316-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining the concentration of voriconazole (VRC) in human plasma.Methods The analysis was conducted using a ZORBAX Eclipse Plus C18 column and the column temperature was 40 ℃.The mobile phase consisted of methanol and water (55∶45).The flow rate was 1 mL · min-1 and the detection was performed at 254 nm.And the internal standard substance was p-Chloroacetanilide.The specificity,lower limit of quantitation (LLOQ),standard curve,precision,accuracy,recovery and stability were investigated.Results Endogenous impurities did not interfere with the determination of the samples in plasma.The standard curve was linear in the range of 0.1-10.0 μg · mL-1 (r =0.999 2),LLOQ was 0.1 μg · mL-1.The RSDs of inter-day and intra-day were all less than 10％ in the plasma and the extraction recovery was 89％-92％.Conclusion This method was simple,sensitive,accurate,efficient and suitable for routine determination of the concentration of VRC in human plasma.
