Study of hepatic ischemia-reperfusion injury on cardiac and renal energy metabolism in rats
10.13699/j.cnki.1001-6821.2018.03.026
- VernacularTitle:肝缺血再灌注损伤对大鼠心肾能量代谢的研究
- Author:
Ming LI
1
;
Feng-Yan MA
;
Ri-Su NA
;
Zhi-Qiang HAN
Author Information
1. 内蒙古民族大学附属医院
- Keywords:
five elements of Mongolian medicine;
mother child relationship of kidney,liver,and heart;
hepatic ischemia -reperfusion;
energy metabolism
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(3):275-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of hepatic ischemia reperfusion injury (IRI)on cardiac and renal energy metabolism in rats.Methods The rats were randomly divided into five groups:the sham operation group,model-0 h group,model-2 h group,model-4 h group,model-6 h group,and ten rats in each group.The hepatic IRI rats model was prepared with non traumatic vascular clamp.The animal were sacrificed immediately after IRI 0,2,4,6 h and samples were collected.The tissue content of Na +-K +-ATPase and Ca2 +-Mg2 +-AT-Pase were detected by enzyme linked immunosorbent assay.Results The Ca2 +-Mg2 +-ATPenzyme of liver tissue in sham operation group,model-2 h group,model-4 h group were (343.84 ± 22.11),(221.88 ±64.15),(235.02 ± 58.84) μmol · g-1;compared with the sham operated group,the differences were statistically significant (all P＜0.05).The Na+-K +-ATP enzyme of renal tissuein sham operation group,model-2 h group,model-4 h group were (79.46 ± 10.84),(67.28 ± 3.39),(69.09 ± 1.47) μmol·g-1.The Ca2+-Mg2+-ATP enzyme of renal tissue in the three groups were (345.68 ± 21.68),(227.39 ± 50.63),(246.38 ± 64.31) μmol · g-1;compared with the sham operated group,the difference was statistically significant (all P ＜ 0.05).The Na +-K +-ATP enzyme of myocardium tissue in sham operation group,model -0 h group,model-2 h group,model-4 h group,model-6 h groupwere (68.16 ± 12.17),(71.92 ± 9.91),(61.62 ±7.96),(59.23 ±3.86),(79.72 ±9.91) μrnol · g-1.The Ca2+-Mg2+-ATP enzyme of myocardium tissue in the five groups were (261.05 ±40.52),(279.13 ±34.12),(243.62 ± 23.59),(244.66 ± 17.79),(291.78 ± 36.38) μmol · g-1;compared with the sham operated group,the differences were statistically significant (all P ＜ 0.05).There were no obvious changes in myocardial tissue energy metabolism indexes.Conclusion From energy metabolism indicators,when there is a hepatic ischemia reperfusion injury,the functions of the heart and kidney are all damaged,but the injury of kidney is earlier than that of myocardium.
