Determination the contents of tenacissoside A and tenacissoside Ⅰ in Marsdeniatena cissima by HPLC
10.13699/j.cnki.1001-6821.2018.02.018
- VernacularTitle:HPLC法测定通关藤药材中2种皂苷含量
- Author:
Qi-Min WANG
1
;
Yin-Ling MA
;
Zhan-Jun DONG
Author Information
1. 河北省人民医院药学部
- Keywords:
HPLC;
Marsdeniatenacissima;
tenacissoside A;
tenacissoside Ⅰ
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(2):158-160,164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for determination of the proportion of tenacissoside A and Ⅰ in Marsdeniatenacissima.Methods Marsdeniatenacissima powder was dissoluted by methanol,treated by ultrasonic,analyzed by HPLC,using Symmetry C1s column(250 mm × 4.6 mm,5 μm).The mobile phase was acetonitrile-0.1％ phosphoric water,gradient elution (0-10 min,32％ A;10-15 min,42％A;15-28 min,50％A).The flow rate was 1 mL · min-1 The detection wavelength was 223 nm.The specificity,standard curve,accuracy,sample recovery,stability and reproducibility of the method were investigated.Results The calibration curves of tenacissoside A were linear in the rangesof 47.2-225.0 μg · mL-1.The average recoveries of three concentrations tenacissoside A were 98.82％,99.24％,99.20％ respectively.The calibration curves of tenacissoside Ⅰ were linear in the ranges of 10.5-50.0 μg · mL-1 The average recoveries of three concentrations tenacissoside Ⅰ were 98.53％,98.32％,98.70％ respectively.The content of tenacissoside A was 5.81-5.96 mg · g-1 The content of tenacissoside Ⅰ was 0.68-0.71 mg · g-1.Conclusion The established method is accurate,simple and reliable,which can be used for the quality concrol of Marsdeniatenacissima.
