Expression of microRNA-181a-5p in serum exosomes of methamphetamine-dependent rats and its biological function analysis
10.13699/j.cnki.1001-6821.2018.01.012
- VernacularTitle:微小RNA-181a-5p在甲基苯丙胺依赖大鼠血清外泌体中的表达及生物信息学分析
- Author:
Han-Cheng LI
1
;
Chan LI
;
Yu-Ting ZHOU
;
Chao-Hua LUO
;
Jing-Ying OU
;
Zhi-Xian MO
Author Information
1. 南方医科大学中医药学院中药药理教研室
- Keywords:
methamphetamine;
conditioned place preference;
microRNA-181a-5p;
serum exosomes;
bioinformatics
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of microRNA (miR)-181 a-5 p in serum exosomes of methamphetamine-dependent rats and healthy rats,and to predict target gene of miR-181a-5p and related bioinformatics analysis.Methods The conditioned place preference (CPP) model was established by methamphetamine:male SD rats were randomly divided into two groups(ten rats in each group):normal group (0.9％ NaCl),and experimental group (methamphetamine 2 mg· kg-1).The experimental group was subcutaneously injected methamphetamine at 8 am,injected saline at 4 pm for four days.The normal group was injected subcutaneously with equal volume of normal saline.In the morning,after the experimental group(normal group)were injected subcutaneously with methamphetamine(saline),they were immediately put in the white side for one hour.In the afternoon,after rats were injected subcutaneously with saline,they were immediately put in the black side for one hour.This training was carried out continuously for 4 days.After 1 day of the last subcutaneous injection of methamphetamine (saline),the conditioned place preference was measured.The residence time and movement distance in the white side were recorded within 15 main.The expression of miR-181a-5p in rats' serum exosomes was detected by RT-qPCR technique.The miR-181a-5p target gene was predicted by Target Scan,miRanda and miRBD.The target gene was analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genomes and Genes pathways (KEGG pathways)with DAVID DataBase.The target gene protein was carried out interactive analysis by STRING online analysis software.Results After CPP training,the residence time (s)and the moving distance (cm)of normal group in the white side were 202.67 ± 28.24 and 3349.26 ± 412.28 respectively;the experimental group were 460.47 ± 66.71 and 4475.97 ± 585.74 respectively.Compared with the normal group,the residence time and the moving distance of the experimental group were significandy increased in the white side(P ＜ 0.01).The expression of miR-181a-5p in serum exosomes in the normal group and the experimental group was 3.39 ± 1.79,10.23 ± 4.32 respectively.Compared with the normal group,the expression of miR-181a-5p in the experimental group had increased significantly(P ＜0.01).The 108 miR-181a-5p target genes were predicted to be rich in biological processes such as neuronal generation and differentiation,gene expression regulation and glutamate transport,and involved in the pathogenesis of protein processing in endoplasmic reticulum.MiR-181a-5p target gene encodes a complex interaction between proteins,including RAD23B,ATXN3,PRKCD,MAP2K1,NCBP1,GABRA1 and so on.They played a core position in the protein-protein interaction (PPI)network.Conclusion miR-181 a-5p may regulate the downstream target protein and participate in the development of methamphetamine addiction by regulating gene expression,glutamate transport and participate in the pathway of protein processing in endoplasmic reticulum.
