Protective effects of Qinjiao on arthralgia rheumatoidarthritis in model rats
10.13699/j.cnki.1001-6821.2018.01.011
- VernacularTitle:秦艽对风湿热痹类风湿关节炎大鼠的保护作用
- Author:
Rong WANG
1
;
Shan SU
;
Shao-Bo SUN
;
Hui-Qin GAO
Author Information
1. 甘肃中医药大学药学院中药学科
- Keywords:
Qinjiao;
compatibility;
rheumatoid arthritis;
ankle pathological change;
nuclear factor kappa B
- From:
The Chinese Journal of Clinical Pharmacology
2018;34(1):41-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of Qinjiao (Gentianae macrophyllae Pall.) in different combinations in wind-damp-heat arthralgia RA model rats and its mechanism.Methods SD rats were randomly divided into eight groups:normal group,disease model group (type Ⅱ collagen),syndrome model group (type Ⅱ collagen + rheumaticfever),positive drug group,Qinjiao group,Qinjiao + Weilingxian (Clematis chinensis Osbeck) group,Qinjiao + Sangjisheng (Taxillus chinensis)group,Qinjiao + Fangji (Stephaniatetrandra) group,each group had ten rats.In the first day of the experiment,the rats in the other groups were given two points on the back,one point at the end of the tail,shaved and intradermally 0.1 mL of collagen Ⅱ (C Ⅱ).In the second day of the experiment,except for the normal group and disease model group,the other groups were reconstructed with the reconstructed artificial climate box for rheumatism and fever model for 11 days.On the 12th day of the experiment,0.1 mL of C Ⅱ emulsion was injected into the left hind paw of the plantar vein to further stimulateand then into the artificial climate box for rheumatoid fever model for 7 days.At the end of the model,the rats in each group were given the corresponding drug by 15 mL · kg-1;the normal group,the disease model group and the syndrome model gronp were given the same amount of saline once a day for 21 days.The first 39 days of the experiment,takingthe ankle joint,the degree of damage of ankle joint was observed by pathological staining with hematoxylin eosin (HE).The expression of nuclear factor kappa B (NF-κB) protein in ankle jointwas determined by immunohistochemical method.The NF-κB expression was semi-quantitative analysis by Western blot.Results The expression of NF-κB protein in the disease model group and the syndrome model group was 170.57 ± 8.93 and 180.02 ± 6.93,respectively.Compared with the expression of NF-κB in the normal group of 105.08 ± 7.97,the difference was statistically significant (all P ＜ 0.05).And the syndrome model group was higher than the disease model group.After administration,compared with others drug groups,the expression of NF-κB protein was 112.37 ± 7.34 in Qinjiao + Fangji group statistically significant (P ＜ 0.05).Compared with the normal model group (0.41 ± 0.16),relative expression of NF-κB protein in the disease model group and the syndrome model group were 0.78 ± 0.12,1.08 ± 0.11,which was significantly higher than that in normal group,and the difference was statistically significant (all P ＜ 0.05).Compared with the syndrome model group,the relative expression of NF-κB protein in Qinjiao + Fangji group was 0.53 ± 0.04,and the difference was statistically significant (P ＜ 0.05).Conclusion Different compatibility of Qinjiao may down expression of NF-κB to protect on RA,among then the combined effect of mild and cold traditional Chinese medicine (TCM) is better than the combined effect of mild and warm TCM,the combined effect of mild and mild TCM.
