Effect of Kangfu Xiaoyan suppositories on uterus tissue inflammatory factors levels in rats with sequela of pelvic inflammatory disease
10.13699/j.cnki.1001-6821.2017.24.017
- VernacularTitle:康妇消炎栓对盆腔炎性疾病后遗症大鼠子宫炎症因子水平的影响研究
- Author:
Ya-Hui WAN
1
;
Kui-Ran LIU
Author Information
1. 中国医科大学附属盛京医院妇产科,沈阳110004
- Keywords:
Kangfu Xiaoyan suppository;
sequela of pelvic inflammatory disease;
inflammatory factor
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(24):2618-2620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Kangfu Xiaoyan suppositories on nuclear factor κB (NF-κB),tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1) levels of rats with sequela of pelvic inflammatory diseases (SPID) Methods Ninety specific pathogen free (SPF) Wistar rats were randomly divided into normal group,sham group,model group,experimental group and positive control group,18 rats in each group.The normal group was given no intervention and normal feeding.Sham group only switch abdominal surgery.The rats of model group,experimental group and positive control group were given foreign body implantation to induce SPID.After 2 weeks,the experimental group were given Kangfu Xiaoyan suppositories 5.04 g · kg-1 d-1,retention enema;and the positive control group were given clindamycin capsules 0.04 g · kg-1 · d-1,intragastric administration for 21 d.The normal group,sham group and model group were not disposed.The uterine swelling rate and swelling inhibition rate were observed in each group.The immunohistochemical method was used to detect the levels of NF-κB,TNF-α and ICAM-1 of rats in each group.Results The uterine swelling rates in sham group,model group,experimental group and positive control group were (10.09 ±5.05)％,(33.98 ± 15.61)％,(7.48 ±8.21)％,(12.31 ±6.67)％.The uterine swelling rate in model group was higher than that in experimental group,with significant difference (P＜0.05).The levels of NF-κB,TNF-α and ICAM-1 in experimental group were(1.33 ±0.82),(1.83 ±0.75),(0.17 ±0.41) ng · L-1,had significant difference with those in model group,which were (4.00 ± 1.79),(5.17 ±2.40),(2.00 ± 1.10) ng · L-1 (P ＜0.05).Conclusion The therapeutic effect of Kangfu Xiaoyan suppositories on SPID rats is related to the inhibition of swelling of uterus and the decrease the levels of inflammatory factors.
