Inhibition of Curcumin analogue FM0807 on pannus formation in rheumatoid arthritis
10.13699/j.cnki.1001-6821.2017.24.016
- VernacularTitle:姜黄素衍生物FM0807抑制佐剂性关节炎血管翳的实验研究
- Author:
Nan-Wen ZHANG
1
,
2
;
Qing-Qing TAN
;
Xia-Zhen LIN
;
Yong-Qing XIE
;
Kai-Mei NIE
;
Xiao-Le CHEN
;
Li-Xian WU
;
Jian-Hua XU
Author Information
1. 福建医科大学药学院,福州 350122
2. 福建省天然药物药理学重点实验室,福州350122
- Keywords:
Curcumin analogue;
rheumatoid arthritis;
pannus;
c-Jun N-terminal kinase
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(24):2615-2617,2641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory effect of Curcumin analogue FM0807 on pannus formation and its possible mechanism.Methods 40 male SD rats were used to establish the adjuvant-induced arthritis rats model,and then randomly divided into model group,control group,test A group,test B group and test C group,8 rats in each group.Another 8 healthy SD rats were used as blank control group.7 days after modeling,test A group,testB group and test C group were given 25,50,100 mg · kg-1 FM0807 through gavage ouce a day;control group was given 0.2 mg · kg-1 dexamethasone intraperitoneal injection,once a day;model group and the blank control group were given 0.9％ NaCl gavage,once a day.The rats in the six groups were treated for 21 days.Observe the content of interleukin-6 (IL-6) and tumor necrosis factor -or (TNF-α) in serum by enzyme-linked immunosorbent assay (ELISA) and determinec-Jun N-terminal kinase (JNK) protein expression by western blotting.Results The contents of TNF-α and IL-6 in serum of test A group,test B group,test C group,control group,model group and blank control group were (106.10 ± 7.15),(88.12 ± 12.75),(67.18 ±11.95),(40.96 ±9.40),(123.76 ±5.05),(18.02±10.97) pg · mL-1and (1.61 ±0.14),(1.48±0.15),(0.73 ± 0.16),(0.41 ± 0.12),(1.88 ± 0.09),(0.27 ± 0.10) pg · mL-1 respectively.JNK protein expression was (7.00 ± 1.22),(3.40 ± 0.54),(2.60 ± 0.55),(1.40 ± 0.55),(7.20 ± 1.30),(1.20 ± 0.27).The differences between test C group and model group were statistically significant (all P ＜ 0.01).Conclusion Curcumin analogue FM0807 may inhibit the formation of pannus and relieve the symptom of rheumatoid arthritis by inhibiting JNK pathway,decreasing the release of inflammatory mediators and reducing inflammatory cell stimulation.
