Interventional effects of Bufrudin on atherosclerosis plaque in ApoE gene knockout mice
10.13699/j.cnki.1001-6821.2017.24.011
- VernacularTitle:菲牛蛭素对载脂蛋白E基因敲除小鼠动脉粥样硬化斑块的干预作用
- Author:
Wu-Fang LAI
1
;
Wei-Guan ZHOU
;
Li-Ying HUANG
;
Xiao-Hui ZHANG
;
Yuan-Hong LI
Author Information
1. 广西医科大学药学院,南宁530021
- Keywords:
Bufrudin;
atherosclerosis;
angiogenesis;
ascular endothelial growth factor
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(24):2595-2598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the interventional effect of Bufrudin on aorta atherosclerosis and angiogenesis in apolipoprotein E gene knockout (ApoE-/-) mice.Methods Male ApoE-/-mice on high fat diet,were randomly divided into three groups:model group,control group,experimental group.Experimental group were given Bufrudin (400 U · kg-1 · d-1),control group were given simvastatin (3 mg · kg-1 · d-1).And C57BL/6J mice fed with common diet was served as normal group.After ten weeks,mice blood was collected and serum levels of triglyceride (TG),total cholesterol(TC),low-density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) concentrations were measured.The expression of CD34 protein,vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR-2) were detected by immunohistochemstry.Results After administraiton,the serum content of TG in model group,control group and experimental group were (1.57 ± 0.12),(1.10 ± 0.21),(0.95 ±0.20) mmol · L-1,respectively;TC in above three groups were (19.93 ± 2.24),(14.47 ± t.30),(11.90 ± 2.38) mmol · L-1,respectively;LDL-C in above three groups were (17.24 ± 1.52),(13.94 ± 1.46),(10.02 ±2.14) mmol · L-1,respectively;HDL-C in above three groups were (4.05 ± 1.81),(4.62 ± 0.50),(3.38 ± 0.24) mmol · L-1,respectively.Compared with model group,the serum content of TG,TC,LDL-C,HDL-C in experimental group were significantly decreased (all P ＜ 0.01).The content of collagen in model group,control group and experimental group were (21.48 ± 2.44)％,(37.12 ± 3.33)％,(51.60 ± 2.47)％;the expression of CD34 protein in above three groups were (11.02 ± 1.64) ％,(6.82 ± 0.66) ％,(4.56 ± 0.59) ％;the expression of VEGf prtein in above three groups were (22.13 ± 2.04) ％,(16.20 ± 0.65) ％,(1 1.16 ± 0.82) ％;and the expression of VEGfR-2 in above three groups were (17.90 ± 0.73)％,(15.11 ± 0.52) ％,(10.56 ± 0.60) ％,respectively.Compared with model group,the content of collagen in experimental group was increased,and the expression of CD34 protein in experimental group was decreased,all about with statistical difference (all P ＜ 0.05).Conclusion Bufrudin attenuate atherosclerosis disease and stabilize the atherosclerotic plaque in ApoE-/-mice likely through suppressing the expression of VEGF and VEGFR-2.
