Effect of dezocine on the expression of glial fibrillary acidic protein of spinal cord in chronic constriction injury rats
10.13699/j.cnki.1001-6821.2017.23.023
- VernacularTitle:地佐辛对坐骨神经慢性压迫性疼痛大鼠脊髓胶质纤维酸性蛋白表达的影响
- Author:
Jian-Ming LIN
1
;
Lin-Lang LUO
;
Yan-Ru ZHENG
;
Wen-Min LIN
;
Wei-Ling LIN
;
Mei-Fen WANG
;
Hang LIN
;
Wei-Fang WU
Author Information
1. 福建省福州儿童医院麻醉科,福州350005
- Keywords:
dezocine;
glial fibrillary acidic protein;
neuropathic pathological pain;
chronic constriction injury;
astrocyte
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(23):2404-2406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of dezocine on spinal cord glial fibrillary acidic protein (GFAP) in rats with chronic constriction injury (CCI) of sciatic nerve as well as its protective effect on sciatic nerve.Methods Thirty two SD rats were randomly divided into sham-operation group,model group,experimental-L,-H groups (with 2.5,10 mg · kg-1 dezocine),eight SD rats in each group.The SD rats in experimental groups were treated with dezocine by intraperitoneal injection once every day from the beginning to seven days after operation;but the SD rats in sham-operation group and model group were treated with normal saline.Seven days after operation,the changes of pathological morphological of sciatic nerve in the four groups were detected.And the expression of GFAP in the four groups was detected by immunofluorescence histochemistry.Results After continuous injection of dezocine for 7 days,the sciatic nerve fibers in experimental-L group rats were swelling,the number of sheath cells decreased,and some of them were dissolved.In experimental-H group,there were a few normal myelin in rats,the swelling of nerve fibers is reduced compared with those in model group,but the number of sheath cells decreased compared with those in sham group,axons can be seen in some myelin sheaths.The expression of glial fibrillary acidic protein of spinal cord in sham-operation group,model group,experimental-L,-H groups were 1.03 ± 0.28,2.08 ±0.55,1.61 ±0.14,1.25 ±0.45.Compared with sham group,the difference in model group was significant (P ＜0.001);compared with model group,the difference in experimental-L,-H groups was significant(P ＜ 0.05,P ＜ 0.01).Conclusion Dezocine can inhibit the neuropathic pathologic pain.The mechanism maybe related to the decrease of the expression of GFAP in spinal cord,the activation of astrocytes,and the relief of the edema of sciatic nerve.
