Effect and its mechanism of Xihuang Pill on hyperplasia of mammary glands in rats
10.13699/j.cnki.1001-6821.2017.23.019
- VernacularTitle:西黄丸对模型大鼠抗乳腺增生作用及其机制研究
- Author:
Xiao-Long LIAN
1
;
Min ZHAO
;
Tao HAN
;
Yu HAN
;
Qiu-Lan WANG
Author Information
1. 甘肃中医药大学药学院,兰州730000
- Keywords:
Xihuang Pill;
hyperplasia of mammary gland;
hormone level;
estradiol;
progesterone
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(23):2390-2393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-hyperplasia of mammary glands effect of Xihuang Pill and its mechanisms in rats.Methods Sixty Wistar rats were equally divided into 6 groups:normal group,model group,control group and low,middle,high dose experimental groups.Except the normal group,the other 5 groups were established the animal models of mammary gland hyperplasia,while modeling,the rats in control group was treated with the dose of 1.8 mg · kg-1 · d-1 tamoxifen,the rats in experimental groups were treated with the dose of 0.54,1.08,2.16 g · kg-1 · d-1 Xihuang Pill;the rats in the normal group and the model group were fed with equal volume of distilled water.The serum levels of estradiol (E2),progesterone (P),prolactin (PRL),testosterone (T) were detected by enzyme league immune assay.The lobular breast density,lobular interstitial density,and alveolar surface area were observed by three-dimensional quantitative method.Results The levels of E2 in normal group,model group,control group,experimental-L group,experimental-M group and experimental-H groups were (44.16 ± 10.63),(65.92 ± 26.98),(47.56 ± 10.95),(50.05 ± 11.84),(50.06 ± 14.30),(48.42 ± 12.48) pg · mL-1;the levels of P in the six groups were (38.26 ± 12.42),(23.24 ± 6.03),(36.77 ± 4.75),(33.11 ± 13.26),(33.04 ± 12.07),(37.75 ± 7.45) ng · mL-1;the levels of PRL in the six groups were (113.23 + 77.83),(253.06 ± 104.90),(159.58 ± 83.25),(168.96 ± 68.76),(130.28 ± 84.44),(142.77 ± 90.36) ng · mL-1,the levels of T in the six groups were (4.01 ± 0.67),(5.46 ± 1.08),(4.24 ± 1.72),(4.69 ± 1.07),(4.64 ± 0.54),(4.29 ± 0.98) ng · mL-1.Compared with normal group,the differences in model group were statistically significant(all P ＜ 0.01).Compared with model group,the difference in experimental groups was statistically significant (P ＜ 0.05,P ＜ 0.01).Lobular breast density in normal group,model group,control group,experimental-L group,experimental-M group and experimental-H groups were (37.82 ±6.37)％,(93.25 ±5.63)％,(40.12 ±8.31)％,(70.33 ±7.56)％,(65.58 ±7.31)％,(50.33±5.24)％;Lobular interstitial density in the six groups were (62.18 ± 6.37)％,(6.75 ± 5.63)％,(59.88 ±8.31)％,(29.67 ±7.56)％,(34.42 ±7.31)％,(49.67 ±5.24)％.Alveolarsurface areain the six groups were (0.92 ±0.19) ％,(2.31 ±0.23)％,(1.11 ±0.18)％,(1.93 ±0.26)％,(1.54 ±0.57)％,(1.20 ±0.30)％.Compared with normal group,the difference in model group was statistically significant(all P ＜0.01).Compared with model group,the difference in experimental groups was statistically significant (P ＜ 0.05,P ＜ 0.01).Conclusion Xihuang Pill can inhibit the hyperplasia of mammary glands induced by estradiol and progesterone in rats.
