Uncertainty evaluation of the determination of lamotrigine in human serum by HPLC
10.13699/j.cnki.1001-6821.2017.22.022
- VernacularTitle:高效液相色谱法测定人血清中拉莫三嗪浓度的不确定度评定
- Author:
Wen-Can HUANG
1
;
Yu-Qing CHEN
;
De-Wei SHANG
;
Xiao-Jia NI
;
Ming ZHANG
;
Zhan-Zhang WANG
;
Hao-Yang LU
;
Xiu-Qing ZHU
;
Shu-Hua DENG
;
Jin-Qing HU
;
Yu-Guan WEN
Author Information
1. 广州医科大学附属脑科医院、广州市惠爱医院药剂科
- Keywords:
HPLC;
lamotrigine;
biological sample analysis;
uncertainty
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(22):2295-2298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the uncertainty in the determination of lamotrigine (LTG) in human serum with high performance liquid chromatography (HPLC).Methods It was analyzed that the influences of various factors for detection results,including weighing,solution preparation,serum extraction,calibration curve fitting repeatability and so on.The expanded uncertainty was evaluated and combined by each component of uncertainty.Results The expanded uncertainty of lamotrigine at low (1.50 μg · mL-1),middle (8.00 μg · mL-1),high (20.00 μg · mL-1) concentrations were UL =0.30,UM =0.53,UH =0.98,respectively (P =95％,k =2).The corresponding test concentration were (1.51 ±0.30),(8.22±0.53),(20.40±0.98) μg · mL-1,respectively.Conclusion The relative uncertainty on the determination of lamotrigine in human serum by HPLC was mainly caused by repeatability,calibration curve fitting and extraction recovery;repeatability and calibration curve fitting were the important factor in influencing the results of low concentrations.
