Protection effect of paeonol on regulation of lipid metabolism in hyperlipidemia mice and its mechanisms
10.13699/j.cnki.1001-6821.2017.22.017
- VernacularTitle:丹皮酚对高脂血症小鼠的脂代谢调节保护作用及其机制
- Author:
Yun CHEN
1
;
Li-Hua KANG
Author Information
1. 重庆医科大学附属第一医院第一分院
- Keywords:
paeonol;
hyslipidemia;
enzyme activity;
lipid synthesis;
oxidative metabolism
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(22):2273-2277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of paeonol on lipid metabolism of hyperlipidemia in mice and clarity the molecule mechanism.Methods According to diet,male C57 mice were randomly divided into normal group,model group,low and high dose experimental groups (paeonol 250,500 mg · kg-1).Each group had ten mice.After 12 weeks,the body weight,visceral fat index,serum lipid and key enzymes were determined.The level of hepatic lipase (HL),lipoprotein lipase (LPL),HMG-CoA reductase enzyme (HMG-CoA RE) were determined by chromatometry.The mRNA expressions of lipid synthesis genes,such as sterol regulatory element-binding protein 1 c (SREBP-1c),fatty acid synthetase (FAS),acetyl CoA carboxylase α (ACCα),3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR),low-density lipoprotein receptor(LDLR),stearoyl-CoA Desaturase-1 (SCD-1) were analyzed by Real -time PCR.Results Feeding with high fat diet for 12 weeks,the total cholesterol(TC),low density lipoprotein cholesterin (LDL-C),high density lipoprotein cholesterin (HDL-C)in model group were (3.87 ± 0.24),(0.87 ± 0.10),(2.23 ± 0.11) mmol · L-1,respectively.Compared with normal group,the difference of the two factors had significantly(P ＜ 0.05,P ＜ 0.01).The TC,LDL-C,HDL-C in experimental-L,experimental-H groups were (3.13 ±0.22),(2.64 ± 0.21) mmol · L-1;(0.62 ± 0.08),(0.59 ±0.07) mmol · L-1;(1.92 ± 0.09),(1.71 ± 0.07) mmol · L-1,respectively.Compared with model group,the difference of the two factors in two experimental groups had significantly (P ＜ 0.05,P ＜ 0.01).The level of HL and LPL in model group were (4.31 ± 0.52),(8.49 ± 1.11) U · mL-1,respectively.Compared with normal group,the difference of the three factors had significantly(P ＜0.05,P ＜0.01).The level of HL and LPL in experimental-L,experimental-H groups were(6.92 ± 1.01),(8.23 ± 1.14) U · mL-1;(11.87 ± 1.19),(15.42 ± 1.75) U · mL-1,respectively.Compared with model group,the difference of the two factors in two experimental groups had significantly (P ＜ 0.05,P ＜0.01).In addition,paeonol can inhibit the activity of HMG-CoA reductase (HMGCR).Compared with model group,the difference of the two factors in two experimental groups had significantly(P ＜ 0.05,P ＜ 0.01).Compared with the normal group,induction of high fat diet of SREBP-1c mRNA 、FAS mRNA 、ACCα mRNA 、HMGCR mRNA and SCD-1 mRNA,the model group was up-regulated 370％,420％,410％,400％,260％ with significantly (all P＜0.01).The experimental group reduced the expression of SREBP-1c mRNA,FAS mRNA,ACCα mRNA,HMGCR mRNA and SCD-1 mRNA in model group were 27.7％,26.9％;57.4％,55.8％ with significandy (all P ＜0.05).Conclusion Paeonol could prevent the obesity and hyperlipidemia,which might be related to the regulation of enzyme activity,the inhibition of lipid synthesis pathway.
