Effect of complement fragments C3f on synthesis of α-smooth muscle actin in human embryonic lung fibroblasts
10.13699/j.cnki.1001-6821.2017.22.014
- VernacularTitle:补体片段C3f对人胚肺成纤维细胞合成α-平滑肌肌动蛋白的影响
- Author:
Wei LIU
1
;
Hai-Xia KONG
;
Juan-Juan CHEN
;
Mao-Ti WEI
;
Shi-Xin WANG
Author Information
1. 天津市第二人民医院检验科、天津市肝病研究所
- Keywords:
α-smooth muscle actin;
transforming growth factor β1;
complement fragment C3f;
human embryonic lung fibroblasts;
myofibroblast
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(22):2262-2264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of complement fragment C3f on synthesis of α-smooth muscle actin (α-SMA) in human embryonic lung fibroblasts (MRC-5).Methods MRC-5 was cultured with C3f of different concentrations (15.6,62.5,250 ng · mL-1) in low,moderate and high dosage experimental groups.And a positive control of 10 ng· mL-1 transforming growth factor-β1 (TGF-β1) and a negative control of 0 ng · mL-1 C3f were also set up for comparative study.Then α-SMA was detected by immunohistochemistry.Results The moderate dose experimental group and positive control group stimulates the synthesis of α-MRC-5 cell SMA with no statistical difference (7.46 ±0.66 vs 7.54 ±0.51,P ＞0.05).It demonstrated that C3f can stimulate the function of MRC-5 on synthesis of α-SMA.With the increase of C3f concentration,the expression of intracellular α-SMA in three doses experimental groups were increased for 4.95 ± 0.78,7.46 ±0.66 and 9.23 ±0.21,respectively,significant differences in three doses experimental groups (all P ＜ 0.05).Moreover,the concentration of C3f was positively correlated with the expression of α-SMA (P ＜ 0.05).C3f has the function of promoting the expression of α-SMA in MRC-5.Conclusion Complement fragment C3f can stimulate the synthesis of α-SMA in MRC-5 cells.
