Effect of ursolic acid on hypertrophy and proliferation of renal glomerular mesangial cells and extracellular matrix production accumulation induced by high glucose
10.13699/j.cnki.1001-6821.2017.21.019
- VernacularTitle:乌索酸对高糖诱导人肾小球系膜细胞增殖肥大及细胞外基质分泌的影响
- Author:
Yuan YUE
1
;
Qiu-Ling FAN
;
Shu-Yan DU
;
Fang YUAN
;
Li XU
;
Lin LI
;
Nan LIU
;
Yi JIANG
;
Li-Ning WANG
Author Information
1. 中国医科大学附属第一医院肾内科
- Keywords:
human mesangial cell;
high glucose;
ursolic acid;
cell hypertrophy;
proliferation;
extracellular matrix
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(21):2158-2161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective role of ursolic acid (UA) on hypertrophy and proliferation of renal glomerrular mesangial cells and extracellular matrix production accumulation induced by high glucose was investigated.Methods The logaritumic growth plase cells were divided into blank control group (5.5 mmol · L-1 glucose),model group (30.0 mmol · L-1 glucose),control group (5.5 mmol · L-1 glucose + 24.5 mmol · L-1 mannitol),test A group (30.0 mmol · L-1 glucose + 0.5 μmol · L-1 ursolic acid),test B group (30.0 mmol · L-1 glucose + 1.0 μmol · L-1ursolic acid),and test C group (30.0 mmol · L-1 glucose + 2.0 μmol · L-1 ursolic acid),and treated for 48 h.Mesangial cell proliferation was detected by MTT method.Cell cycle and cell hypertrophy were tested by PI single dye flow cytometry.Reverse transcription-polymerase chain reaction and Western Blot were used to evaluate transforming growth factor-β1 (TGF-β1),fibronectin (FN) mRNA and protein expression.Results After 48 h,the cell proliferation rates of blank control group,model group,control group,test A group,test B group,and test C group were (3.66 ± 0.32),(4.33 ±0.23),(3.50 ±0.22),(3.93 ±0.11),(3.59 ±0.06) and (0.67 ±0.16).The comparison between the model group and the blank control group was statistically significant(P ＜ 0.05);the comparisons between the test B,C groups and the model group were statistically significant (all P ＜ 0.05);and there were no statistically significant differences between the test A group and the blank control and control groups (all P ＞ 0.05).Most of the cells in the test C group were dead,and the test B group was set up to be the final test group.The main indexes in blank control group,model group,control group and test group were compared:TGF-β1 mRNA were (24.80 ± 0.90),(29.89±1.06),(23.72±0.87) and (25.06 ±0.11),FN mRNA were (42.03 ±0.57),(54.55 ± 1.20),(42.72 ± 0.40) and (45.32 ± 0.51),TGF-β1 protein expression were (54.92 ± 3.28),(125.93 ± 3.58),(25.11 ± 0.51) and (101.23 ± 7.55),FN protein expression were (46.44 ± 7.90),(66.13 ± 13.10),(34.98 ± 5.52) and (28.70 ± 5.70).The comparisons between the model group and the blank control and test groups were statistically significant (all P ＜ 0.05).Conclusion UA can alleviate the injury ofmesangial cell induced by high glucose by inhibiting mesangial cell proliferation,hypertrophy and extracellular matrix deposition.
