Comparison the methods of LC-MS/MS and 2D-LC/UV for determination of olanzapine in human serum
10.13699/j.cnki.1001-6821.2017.20.021
- VernacularTitle:LC-MS/MS与2D-LC/UV法测定奥氮平血药浓度的比较
- Author:
Yu-Qing CHEN
1
;
Yan-Ping ZHOU
;
Jing TENG
;
Feng WANG
;
Yu-Guan WEN
Author Information
1. 广州医科大学附属脑科医院、广州市惠爱医院药学部
- Keywords:
2D-LC/UV;
LC-MS/MS;
olanzapine;
serum concentration;
consistency
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2068-2072
- CountryChina
- Language:Chinese
-
Abstract:
Objective The LC-MS/MS method and 2D-LC/UV method were used to compared the consistency and correlation of olanzapine serum concentration.Methods Quality control samples and 52 serum samples of olanzapine were detected by LC-MS/MS and 2D-LC/UV method respectively.The consistency of results was compared by Bland-Altman method,and the correlation was analyzed by Pearson correlation analysis and Passing-Bablok regression.Results According to the Bland-Altman method,the percentage of the limit can be obtained:96.15％ LoA =(-1.53,3.81).And 96.15％ difference value was within the limit and the consistency of the two methods was good.According to the Pearson linear regression analysis,Passing-Bablok regression equation was OLZLC-MS/MS =1.02 × OLZ2D-LC/UV-1.18 and correlation coefficient was 0.999 (P ＜ 0.01) with good correlation.Compared to the middle and high serum concentration,the relative deviation testes by two methods in low serum concentration(＜ 15 ng · mL-1) was larger.Conclusion The 2D-LC/ UV method and LC-MS/MS method have the good consistency and correlation in detecting olanzapine serum concentration,which can be used for conventional therapeutic drug monitoring(TDM).
