Determination of bicalutamide concentration in human plasma by HPLC-MS/MS
10.13699/j.cnki.1001-6821.2017.20.020
- VernacularTitle:高效液相色谱-质谱联用法测定人血浆中比卡鲁胺的浓度
- Author:
Guo-Fang BI
1
;
Ying-Fu ZHANG
;
Yuan-Yuan ZHANG
;
Li-Jun ZHANG
;
Xiao-Fang WANG
;
Yuan-Yuan LI
;
Long-Mei CHENG
;
Heng-Yan QU
Author Information
1. 军事医学科学院附属医院临床药理学研究室
- Keywords:
bicalutamide;
HPLC-MS/MS;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2065-2067
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the bicalutamide concentration in human plasma by established HPLC-MS/MS method.Methods The plasma was treated with protein precipitation,Bicalutamide-d4 was used as internal standard,chromatographic column:Agilent ZORBAX SB-C18 column (2.1 mm ×S0.0 mm,5 μm),column temperature:20 ℃,mobile phase:methanol-0.1％ formic acid and 2 mmol · L-1 ammonium formate,isocratic elution,flow rate:0.35 mL · min-1,mass concentration of the sample was measured with negative ion scan and multiple reaction monitoring mode.The specificity,lower limit of quantitation and standard curve,precision and recovery rate and stability as well as the matrix effect were investigated.Results The standard curve of bicalutamide was y =6.82 × 10-3x + 1.33 × 10-2 (r =0.999 1),and has good linear relationship in 10-1500 ng · mL-1,the lowest concentration was 10 ng · mL-1.Intra-day and inter-day RSD of blood samples were less than 15％,the average recovery was ＞ 95％,and stability was good.Conclusion The method was simple,rapid,sensitive and accurate,specific,suitable for the determination of bicalutamide concentration in human plasma.
