Protective effect of ethanolic extract of Saussurea.graminea Dunn against mice liver injury induced by carbon tetrachloride
10.13699/j.cnki.1001-6821.2017.20.018
- VernacularTitle:禾叶风毛菊醇提物对四氯化碳致小鼠肝损伤的保护作用
- Author:
Ya-Qi RAO
1
;
Li ZHANG
;
Jin-Xiang ZENG
;
Ji-Xiao ZHU
;
Guang-Ming LUO
;
Guo-Yue ZHONG
Author Information
1. 江西中医药大学
- Keywords:
ethanolic extract of Saussurea.graminea Dunn;
carbon tetrachloride;
liver injury
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2058-2061
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of ethanolic extract of Saussurea.graminea Dunn (EESGD) on mice liver injury induced by carbon tetrachloride (CCl4).Methods Mice were randomly divided into 6 groups,such as normal group,control group (dimethyl dicarboxylate biphenyl,0.15 g · kg-1),model group,experimental-H,experimental-M,and experimental-L groups (EESGD:4,2,1 g · kg-1).The liver injury model was induced by intraperitoneal injection of 0.15％ CCl4 peanut oil solution.At the time of 16 hours after modeling,levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were determined by performance rate method.The levels of tumor necrosis factor(TNF-α) and interleukin-6 (IL-6) in serum were detectioned by Enzymelinked immunosorbent assay (ELISA) method.The liver samples were collected for the examination of the contents of malondial dehyde (MDA),glutathione (GSH) and superoxidase dismutase (SOD) in liver homogenate used by colorimetry method.Results ALT in normal group,control group,model group,experimental-H,experimental-M,and experimental-L groups of EESGD were (14.90---3.35),(28.11 ± 12.55),(47.38 ± 20.15),(30.22 ± 16.63),(32.67 ± 11.67),(43.38 ± 25.91) U · L-1;AST were (97.75 ± 11.96),(99.00 ± 9.50),(129.86 ± 17.67),(94.50 ± 11.56),(96.86± 13.06),(112.29 ± 15.30) U·L-1 in above six groups.TNF-α were (382.92 ± 82.01),(626.79 ± 142.09),(914.69 ± 157.98),(425.89 ± 149.40),(756.87 ±76.49),(654.79 ± 131.54) pg · mL-1in above six groups.IL-6 were (18.44±2.23),(24.14 ± 9.01),(58.13 ± 8.68),(34.20 ± 11.65),(34.73 ±13.40),(42.15 ±19.56) pg · mL-1 in above six groups.SOD were (91.62 ±3.92),(85.06 ±7.94),(48.86±2.3),(86.15 ±6.05),(66.04 ±7.83),(54.74 ±7.77) U · mg-1in above six groups.MDA were (0.31 ±0.04),(0.68 ±0.14),(0.83 ±0.08),(0.58 ±0.16),(0.77 ± 0.08),(0.82 ±0.17) nmol · mg-1in above six groups.GSH were (10.36 ± 0.38),(9.70 ± 0.56),(8.00 ± 0.43),(9.92 ± 0.73),(8.74 ± 0.72),(8.78 ± 0.65) mg · g-1 in above six groups.Compared with the model group,experimental-H and experimental-M groups significantly decreased ALT,AST in blood serum(P ＜ 0.05,P ＜ 0.01).All dose experimental groups evidently depressed TNF-α and IL-6 in blood serum with significantly (P ＜ 0.05,P ＜ 0.01).All dose experimental groups remarkably advanced the activity of SOD and GSH in liver homogenate with significantly (P ＜ 0.05,P ＜ 0.01).The experimental-H group significantly decreased the activity of MDA in liver homogenate (P ＜ 0.01).Conclusion EESGD have a certain protective effect on mice liver injury induced by CCl4.
