Sensitivity of Oridonin on temozolomide through enhances the U251 and via down-regulating nuclear factor-κB pathway
10.13699/j.cnki.1001-6821.2017.20.015
- VernacularTitle:冬凌草甲素通过下调控核因子κB通路增强U251对替莫唑胺的敏感性
- Author:
Jian BAI
1
;
Li-Min WANG
;
Jun LIU
;
Da-Yong LIN
;
Lan-Ying MIAO
;
Hong LIU
;
Li-Yan ZHANG
Author Information
1. 中国人民解放军第三○九医院器官移植研究所
- Keywords:
Oridonin;
nuclear factor-κB;
glioma;
apoptosis;
temozolomide
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2047-2050
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the timorazolamide sensitivity for U251 after the pretreatment by Oridonin.Methods All experiments were divided into 5 groups including control group (not drugs) and extreme low,low,medium,and high concentrations (Oridonin:10,20,40,80 μmol · L-1) groups.U251 cells in all groups were cultured in 24 h,48 h and 72 h.At different time points,U251 cells' proliferation and nuclear factor-κB (NF-κB) protein expression in all groups were detected by Western blot.After culturing U251 cells in medium group and high group for 72 h,cells in the two groups continued to be incubated in extreme low,low,medium,and high concentrations (Temozolomide:50,100,200,400 μmol · L-1) for 24 h and 48 h.Apoptosis ratio in the two groups were detected by flow cytometer.Results After culturing 48 h and 72 h,relative expression of NF-κB in medium group and high group were 0.67±0.11,0.55 ±0.21;0.45 ±0.12,0.51 ±0.17 respectively,compared with the control group,the difference had statisticalsignificantly (all P ＜ 0.05).After culturing 72 h in medium group and high group,U251 cells continued to be incubated in 4 different concentrations temozolomide groups for 48 h,and apoptosis ratio were(50.13 ±5.44) ％,(55.34 ±5.93) ％,(55.58 ± 4.92)％,(57.51 ± 4.89)％;(55.67 ± 3.96)％,(60.34 ± 6.89)％,(59.77 ± 4.71)％,(63.45-± 5.67) ％,compared with the control group,the difference had statistical significantly (all P ＜ 0.05).Conclusion The NF-κB protein expression of U251 cell can inhibited by Oridonin,and the drug sensitivity of U251 cells can be enhanced by pretreatment of temozolomide.
