Effects of overexpression of α 2,3-sialyltransferase I on invasion of breast cancer MDA-MB-231 cells
10.13699/j.cnki.1001-6821.2017.20.013
- VernacularTitle:过表达α2,3-唾液酸转移酶基因对乳腺癌MDA-MB-231细胞侵袭能力的影响
- Author:
Wen-Ju LIANG
1
;
Qin WANG
;
Ying LIU
;
Shang-Guo SUN
;
Hong-Xia CUI
Author Information
1. 齐齐哈尔医学院药学院
- Keywords:
overexpression;
α 2,3-sialyltransferase;
transfection;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2039-2042
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of overexpression of α 2,3-sialyltransferase (ST3Gal Ⅲ) on abilities of invasion in breast cancer cells MDA-MB-231.Methods ST3Gal Ⅲ gene lentiviral expression vector was transfected into MDA-MB-231 cells.Transfected cells were divided into 3 groups:blank group (Mock cells,M),control group (Parent cells P),experimental group (ST3Gal Ⅲ ST3).The expression of ST3Gal Ⅲ mRNA and protein were detected by Quantitative Real-time PCR(RT-PCR) and Western blot.The content of α2,3-sialic acids was analyzed by flow cytometry.The ability of migration and invasion in MDA-MB-231 cells were detected by cell adhesion assay and Transwell assay,respectively.Results The expression levels of ST3Gal Ⅲ mRNA and protein examined by RT-PCR and Western blot in control group and experimental group were 0.91 ± 0.04 and 1.85 ± 0.08 respectively.The expression levels in experimental group were 1.84-fold and 1.76-fold higher than control group(P ＜ 0.05).The amount of SLeX on the cell surface in experimental group was 92.86 ± 4.41 and was 1.61-fold higher than control group,which were significantly increased compared with that in the blank group and control group (P ＜ 0.05).OD570nm values of the adhesion assay in the in the blank group,the control group and the experimental group were 0.32 ± 0.02,0.29 ± 0.01 and 0.46 ±0.03,respectively,while those of the invasion assay were 0.93 ±0.02,0.81 ±0.01 and 1.46 ±0.13,and there was significant difference between the experimental group and the control group (P ＜ 0.05).Conclusion Overexpression of ST3Gal Ⅲ gene could enhance the invasion ability of MDA-MB-231 cells.
