Study on the role of microRNA-195 in transforming growth factor-β/Smad signal pathway and the fibrosis mechanism
10.13699/j.cnki.1001-6821.2017.20.012
- VernacularTitle:微小RNA-195调控转化生长因子-β/Smad信号通路影响肝纤维化机制研究
- Author:
Yu-Tao MA
1
;
Yang HE
;
Pei-Pei MENG
;
Shan-Ru ZUO
;
Ren GUO
;
Shi-Kun LIU
Author Information
1. 中南大学药学院
- Keywords:
miRNA-195;
Smad7;
transforming growth factor-β1;
rat hepatic stellate cell;
hepatic fibrosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(20):2035-2038
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of miRNA-195 (miR-195) on Smad7 expression and rat hepatic stellate cells line (HSC-T6) activation incuced by transforming growth factor-β1 (TGF-β1).Methods HSC-T6 were cultured in vitro,with 5 ng · mL-1 TGF-β1 as an injury factor simulating the rats liver fibrosis models.The cells divided into six groups:control group,model group (TGF-β1 group),miR-195 mimic experimental group,miR-195.mimic NC experimental group,miR-195 inhibitor experimental group and miR-195 inhibitor NC experimental group.After the HSC-T6 treated with 5 ng · mL-1 TGF-β1for 24,48,72 h,the mRNA expression of miR-195,Smad7,and α-SMA was detected by Real-time PCR.The protein expression of Smad7 was detected by Western blot.Results Under the induced by TGF-β1,the expression of miR-195 increased gradually (P ＜ 0.01),the expression of Smad7 showed a decreasing trend (P ＜ 0.05),and the expression of oα-SMA was gradually upregulated (P ＜ 0.01) with time.Compared with the model group,miR-195 mimic could promote activation of HSC-T6 induced by TGF-β1,and increase miR-195,α-SMA mRNA expression (all P ＜0.01),as well as reduce Smad7 mRNA and protein expression (all P ＜0.01);while miR-195 inhibitor could inhibit activation of HSC-T6 induced by TGF-β1,decrease miR-195,α-SMA mRNA expression (all P ＜ 0.01),as well as upregulate Smad7 mRNA and protein expression (all P ＜ 0.01).Conclusion miR-195 is involved in pro-activation of HSC-T6 by inhibiting Smad7 expression.
