Effects of Hydroxysafflor yellow A on the T lymphocytes dysfunction of human peripheral blood induced by lipopolysaccharide
10.13699/j.cnki.1001-6821.2017.19.026
- VernacularTitle:羟基红花黄色素A对脂多糖诱导的人外周血T淋巴细胞损伤的影响
- Author:
Jin-Ping WANG
1
;
Run-Hua CHEN
;
Han-Wei WU
;
Ze-Yu HUANG
;
Pei-Yan ZHAO
;
Ping WANG
;
Jian-Long WU
Author Information
1. 深圳市第二人民医院药学部
- Keywords:
Hydroxysafflor yellow A;
T lymphocyte;
proliferation;
mitochondrial injury
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(19):1942-1945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Hydroxysafflor yellow A (HSYA) on T lymphocytes proliferation and mitochondrial membrane injury of human peripheral blood induced by lipopolysaccharide.Methods Human T lymphocytes were isolated from peripheral blood.The T lymphocyte suspension was divided averagely into 5 groups as followings:blank control group (without any drug and cell),positive control group (lipopolysaccharides 80 μg · mL-1),low,middle,high concentration test groups (200,400,800 μmol · L-1 HSYA + lipopolysaccharides 80 μg· mL-1)-Cell counting kit-8 (CCK-8) was used to measure the proliferation of T cells,and mitochondrial membrane was measured by flow cytometry.Results The cell viability in positive control group and low,middle,high concentration test groups were 50％,63％,90％,99％.The cell viability in middle,high concentration test groups had significant difference with positive control group and low concentration test group (P ＜ 0.05).The frequency of mitochondrial membrane injury in positive control group and low,middle,high concentration test groups were 19.2％,17.3％,13.0％,10.4％,the frequency of mitochondrial membrane injury decreased the most significant in high concentration test group.Conclusion HSYA was effective in promoting the proliferation of T lymphocytes,which inhibited by lipopolysaccharides,and ameliorated lipopolysaccharides-induced mitochondrial membrane injury.Thus,HSYA might be useful as a potential therapeutic medication for treating sepsis.
