Effect of lung protection of remifentanil in rats with acute severe hemorrhagic shock
10.13699/j.cnki.1001-6821.2017.19.022
- VernacularTitle:瑞芬太尼对急性重度失血性休克大鼠的肺保护作用研究
- Author:
Chuan-Yu BAO
1
;
Long-He LI
;
Duo-Hu WU
;
Yong-Ping ZOU
;
Jian MAO
Author Information
1. 海南省人民医院急诊科
- Keywords:
acute severe hemorrhagic shock;
remifentanil;
lung protection
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(19):1926-1929
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the lung protection of remifentanil in rats with acute severe hemorrhagic shock (ASHS).Methods A total of 50 healthy adult SD rats were selected and randomly divided into model group,sham-operation group and low,middle,high dose test groups,10 rats in each group.Model group were ASHS model rats.Other groups were intravenous given injection with loading-dose of remifentanil 1 μg · kg-1,then low,middle and high dose test groups were intravenous infusion with 0.2,0.6,1.2 μg · kg-1 · min-1 remifentanil.The lung hilus in model group and sham-operation group were dissociate without blockage,while model group and sham-operation group were infused with 0.9％ NaC1 as the same dose of high-dose test group.The injuries of lung tissues in rats were judged by pathological examination.The wet to dry weight ratio (W/D) of lung tissue,malondialdehyde (MDA),activity of superoxide dismutase (SOD),content of nitrogen oxide (NO) were detected in each group.Results The W/D,MDA,SOD,NO in model group were 2.97 ± 0.17,(3.45 ± 1.48) nmol · mg-1 prot,(11.73 ± 1.78)U · mg-1 prot,(0.13 ±0.03)μmol · mg-1 prot,had no significant difference with those in low dose test group,which were 2.71 ± 0.12,(2.90 ± 1.03) nmol · mg-1 prot,(14.44 ± 1.55) U · mg-1 prot,(0.16 ± 0.05) μmol · mg-1 prot (all P ＞ 0.05).The W/D,MDA,SOD,NO in middle-dose test group were 2.33 ±0.16,(2.29 ±0.72) nmol · mg-1 prot,(15.59 ± 1.80)U · mg-1prot,(0.19 ±0.05) μmol· mg-1prot,and those in high-dose test group were 2.08 ± 0.07,(1.81 ± 0.64)nmol · mg-1 prot,(17.47 ± 1.10)U · mg-1prot,(0.26 ±0.08) μmol · mg-1prot,these indexes in middle-dose and high-dose test groups had significant difference with model group (all P ＜ 0.05).Conclusion Remifentanil can inhibit lipid peroxidation,reduce injury of lung tissue,so as to protect the lung of ASHS rats.
