Effects of thapsigargin on the apoptosis in mouse lung fibroblast stimulated by transforming growth factor-β1
10.13699/j.cnki.1001-6821.2017.19.019
- VernacularTitle:毒胡萝卜素对转化生长因子β1刺激的小鼠肺成纤维细胞凋亡的影响
- Author:
Yu YAN
1
;
Si-Qi MA
;
Qian XIAO
;
Yin-E QU
;
Yong-Hong XIAO
Author Information
1. 华北理工大学临床医学院
- Keywords:
lung fibroblasts;
thapsigargin;
cell cycle;
apoptosis;
cysteinyl aspartate specific proteinase
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(19):1915-1917
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of thapsigargin on the cell cycle,apoptosis and cysteinyl aspartate specific proteinase (Caspase-12) protein expression in mouse lung fibroblast stimulated transforming growth factor-β1 (TGF-β1).Methods Mice lung fibroblast lines frozen in liquid nitrogen were recovered and cultured.Experiment was divided into three groups:the control group,model group and experimental group.RPMI-1640 medium was only add in the control group,5 ng · mL-1 TGF-β1 was add in the model group;while in experimental group,5 ng · mL-1 TGF-β1 was add for 24 hours,then 4 pmol · L-1 thapsigargin was add.After 24 h,the cell cycle and apoptosis were detected by flow cytometry instrument.The protein expression of caspase-12 was examined using the immunohistochemical method.Results In control group,model group and experimental group,the proportion of G1 phase cells were (58.72 ±8.35)％,(52.13 ±7.61)％ and (73.02 ±9.32)％,which of S phase cells were (29.50 ± 4.24) ％,(40.54 ± 6.31) ％ and (21.66 ± 4.17) ％,G2 phase cells was (11.75 ± 0.96) ％,(7.62 ± 3.22)％ and (6.09 ± 2.11)％ respectively,the differences had statistical significance in G1 phase,S phase and G2 phase among groups (all P ＜ 0.05),while there was no significant difference in G2 phase between experimental group and model group (P ＞ 0.05).The apoptosis rate in control group,model group and experimenta group were 6.73％,5.03％ and 21.53％ respectively,the protein expression of Caspase-12 were 3356.45 ±205.74,2645.56 ± 168.36 and 5424.76 ± 241.79,the differences in above two indicators were statistical significant (all P ＜ 0.05).Conclusion Thapsigargin could promote the apoptosis of lung fibroblast and increase the protein expression of caspase-12.
