Expression and significance of tumor necrosis factor-α in alcoholic liver injury of mice
10.13699/j.cnki.1001-6821.2017.19.018
- VernacularTitle:肿瘤坏死因子-α在小鼠酒精性肝损伤过程中的表达及意义
- Author:
Xiao-Ping LI
1
;
San-Qiang LI
;
Dong-Mei WANG
;
Xin-Jie QIAO
;
Hua-Jie LU
;
Miao-Shui BAI
Author Information
1. 河南科技大学医学院肝脏损伤与修复分子医学重点实验室
- Keywords:
alcohol lavage;
alcoholic liver injury;
tumor necrosis factor-α;
Western blotting
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(19):1912-1914
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes and effects of tumor necrosis factor (TNF-α) expression in mice with alcohol-induced liver injury.Methods According to the principle of random distribution,50 mice were classified into two groups,including the normal control group(n =40) and the model group (n =10).Administered with alcohol of 56 degrees (15 mL · kg-1) for 1 week and 2,3,4 weeks to induce liver injury.All mice in control group were executed to obtain the eye blood and the liver tissue in 0 (control group),1 week and 2,3,4 weeks.The aspartate transaminase (AST) activities in mice were detected by Reitman-Frankel methods.With the detection of Western blotting,the expressions of TNF-α were measured in the mice of control group and model group in the process of alcohol-induced liver injury.Results The activity of AST in liver of mice at 0 (control group),1 week and 2,3,4 weeks were (112 ±22),(126 ±24),(967 ±30),(1010 ±35),(206 ±23) U · L-1,respectively.Compared with control group,the differences were significantly (all P ＜ 0.05).Compared with the control group,AST activities in the model group increased slowly in the first weeks,then rose rapidly in the second week,reached the peak in the third week,began to decline in the fourth week,but still higher than the control group.TNF-α level at 0,1 week and 2,3,4 weeks were 1.15 ±0.12,1.10±0.08,2.13 ±0.16,2.16 ±0.15,1.16 ±0.11 respectively.Compared with the control group,the differences were significantly(all P ＜0.01).Compared with the control group,the expression of TNF-α in the model group gradually rose from the first week to the third week,then fell close to normal level across time.Condusion The expression of TNF-α had obvious varieties in the process of alcoholic hepatic injury in mice,which demonstrates TNF-α may have significant impact on the injury and restoration of liver tissue.
