Association of cytochrome P450 2C9 genetic polymorphisms with susceptibility to epilepsy and serum concentration of valproate acid
10.13699/j.cnki.1001-6821.2017.19.016
- VernacularTitle:细胞色素P450 2C9基因多态性与癫痫易感性及其与丙戊酸血清浓度相关性
- Author:
Sheng-Ju YIN
1
;
Xin-Sheng XU
;
Xin-Feng LIU
;
Shi-Liang CHENG
;
Cui-Hua WANG
Author Information
1. 山东省交通医院检验科
- Keywords:
epilepsy;
cytochrome P450 2C9;
valproate acid;
serum concentration
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(19):1906-1908
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of cytochrome P450 2C9 (CYP2C9 * 3) (1075A ＞ C) gene polymorphisms with the susceptibility to epilepsy and the serum concentration of valproate acid (VPA).Methods DNA was extracted from peripheral blood of 245 healthy subjects and 191 patients with epilepsy.The CYP2C9 * 3 genotype was detected by sequenom mass array method.The steady-state serum concentration of VPA was determined by fluorescence polarization immunoassay.Results The frequencies of CYP2C9 * 3 alleles in patients with epilepsy and healthy subjects were 4.45％ and 2.04％,respectively,and the difference was statistically significant (P ＜ 0.05).The C allele frequency odds ratio was 2.23,C allele increased the risk of epilepsy (P ＜0.05).The steady-state VPA concentration in patients with CYP2C9 * 1/* 1 genotype and patients with CYP2C9 * 1/* 3 genotype were (55.90±21.11) and (67.75 ±21.36)μg· mL-1,respectively,and the difference was statistically significant (P ＜ 0.05).Conclusion CYP2C9 * 3 significantly increases the risk to develop epilepsy and C allele is a susceptible allele for epilepsy.CYP2C9 * 3 polymorphisms are associated with serum concentrations of VPA in epilepsy patients.
