Effect of curcumin on the viavility migration and invasion of breast cancer cells and its mechanism
10.13699/j.cnki.1001-6821.2017.18.017
- VernacularTitle:姜黄素对乳腺癌细胞的增殖与迁移及侵袭的影响及其作用机制
- Author:
Qiao-Lin WANG
1
;
Na-Na HE
;
Ming LU
;
Ning WANG
;
Yin CHEN
Author Information
1. 新疆医科大学附属中医医院肿瘤一科
- Keywords:
curcumin;
breast cancer;
viability;
migration;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(18):1793-1796
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and the mechanisms of curcumin on in vitro viability,migration and invasion of human breast cancer cell line.Methods The test group (the concentrations of curcumin were 5,10,20 μg · mL-1,respectively)and control group were set in experiment.The inhibition effect of curcumin on the viability of MDA-MB-231 was determined with methyl thiazolyl tetrazolium (MTT).The apoptosis rate was determined with TUNEL assay.Themigration rate was determined with wounding assay.The invasion number of MDA-MB-231 was determined with tranwell assay.The expression of phosphorP38 was determined with Western blot.Results Curcumin suppressed MDA-MB-231 cell viability in a concentration-dependent manner when compared to the control.In high-dose group,cell viability after 48 h of curcumin treatment was decreased 49.17％ compared to control group;In medium-dose group,cell viability after 48 h of treatment was decreased 67.63％.In medium-dose group,the apoptosis rate after 48 h of curcumin treatment was increased 6.44％ compared to control group (0.38％);the apoptosis rate 48 h of curcumin treatment was increased 24.22％ in high-dose group.In medium-dose group,the migration rate after 48 h of curcumin treatment was decreased 55.33％ compared to control group;the migration rate 48 h of curcumin treatment was decreased 40.67％ in high-dose group.In medium-dose group,the invasion number after 48 h of curcumin treatment was decreased (37 ± 7) cell,compared to control group (104 ± 8) cell;the invasion number 48 h of curcumin treatment was decreased (25± 9) cell in high-dose group.In medium-dose group,the expression of phosphor-P38 after 48 h of curcumin treatment was decreased 57.63％ compared to control group;the expression of phosphor-P38 was decreased 42.51％ in high-dose group.Conclusion Curcumin suppresses human liver cancer cell MDA-MB-231 viability,migration and invasion by suppressing the expression of phosphorylation P38.
