Study of regulation mechanism of adrenal gland on rat model with cervical spondylosis of vretebral artery type based on gene expression profiles
10.13699/j.cnki.1001-6821.2017.18.016
- VernacularTitle:基于基因表达谱分析肾上腺对椎动脉型颈椎病模型大鼠的调控机制研究
- Author:
Min SONG
1
;
Yan-Long GONG
;
Tao LIU
;
Jian-Hong LIU
;
Wan-Tao DONG
;
Ling-Tong ZHOU
;
Kai HUANG
;
Hong-Yan HOU
Author Information
1. 甘肃中医药大学中医临床学院
- Keywords:
gene chip;
adrenal gland;
cervical spondylosis of vretebral artery type;
gene expression profile
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(18):1789-1792
- CountryChina
- Language:Chinese
-
Abstract:
Objective Tio Analyze the changes of gene expression profile of adrenal tissue in cervical spondylosis of vretebral artery type (CSA) of model rats and exploring the mechanism of adrenal gland control on CSA.Methods Wistar rats were randomly divided into model group and normal group.The CSA model was established by composite modeling method.The total RNA was extracted from the adrenal gland after the model was successfully obtained.The gene expression profile was detected by whole gene chip,Gene Ontology (GO),Kyoto Encyclopedia of Genes and Genomes(KEGG) signal pathway analysis.Results Compared with the normal group,the difference gene expression profile analysis showed that the total number of differential genes was 367 (| fold change [＞ 2,P ＜0.05),among which 236 were up-regulated and 131 were down-regulated.The enrichment of gene GO functions a total of 2843,involving the regulation of cell cycle,the regulation of stress stimulation,the regulation of fibroblast proliferation,the regulation of endothelial cell proliferation,etc.Participating in the regulation of signaling pathways about 135,including mitogen-activated protein kinase (MAPK) signal transduction pathway,phosphatidylinositol 3 kinase/protein kinase B (PI3 K/Akt) signal path,Hedgehog signal path,Wingless and Int(Wnt) signal pathway and so on.Conclusion The regulation of CSA on adrenal gland is mainly to regulate the proliferation and migration of vascular endothelial cells and vascular smooth muscle cells,adhesion molecules,fibroblasts,and the stress response to stimulation.
