Effect of enalapril folic acid tablet on peripheral blood inflammatory factors in type H hypertensive of rats
10.13699/j.cnki.1001-6821.2017.18.014
- VernacularTitle:依那普利叶酸片对H型高血压大鼠外周血炎症因子及颈动脉结构的影响
- Author:
Zheng OUYANG
1
;
Hong-Yu TANG
;
Ai-Min WANG
;
Qing-Fang LIU
;
Zheng LIU
;
Chen WEI
Author Information
1. 长沙市第一医院神经内科
- Keywords:
type H hypertension;
enalapril folic acid tablets;
inflammatory factor;
carotid artery structure
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(18):1780-1784
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of enalapril folic acid on peripheral blood inflammatory factors in type H hypertensive of rats.Methods Sixty Wistar Spontaneous hypertension male rats were randomly divided into control group,model group,enalapril group and enalapril folic acid group,15 rats in each group.Type H hypertensive rat model were established by feeding with 3％ methionine in model group,enalapril group and enalapril folic acid group.And enalapril folic acid group were given 10 mL · kg-1 enalapril maleate folic acid solution,enalapril group were given 10 mL · kg-1 enalapril solution,and the model group were given 10 mL · kg-1 double distilled water,and 4 groups were intervened for 8 weeks everyday.The serum levels of homocysteine (Hcy),serum tumor necrosis factor alpha (TNF-α),hypersensitivity C reactive protein (hs-CRP)and soluble OX40 ligand(OX40L) were measured by enzyme-linked immunosorbent assay.The nitric oxide(NO)level were measured by End-point assay.The structure of carotid artery was observed under microscope.Results Compared with Hcy(64.22 ±6.82) μ mol · L-1 and NO(41.45 ±5.25) μmol · L-1 in the enalapril group,levels of Hcy(19.75 ±2.14) μmol · L-1 were lower,and NO(57.68 ±6.43) μmol · L-1were higher in the enalapril folic acid group with significantly (P ＜ 0.05).Compared with TNF-α (72.72 ± 6.89) pg · mL-1,hs-CRP (1.15 ±0.12) pg · mL-1,OX40L(5.24 ±0.47) ng · mL-1in the enalapril group,levels of TNF-α(58.18 ±5.76)pg · mL-1,hs-CRP(0.86-±0.11) pg · mL-1,OX40L(3.03 ±0.39) ng · mL-1 were lower in the enalapril folic acid group with significantly(P ＜0.05).Compared with the carotid artery IMT (61.14±7.73) μm,and IMCSA levels(0.12 ± 0.02) mm2 in the enalapril group,the carotid artery IMT (52.74±6.29)μm,and IMCSA (0.10 ±0.01) mm2 in the enalapril folic acid group were lower with significantly(P ＜ 0.05).Compared with the levels of carotid artery LD(951.38 ± 6.08) μm in the enalapril group,the levels of carotid LD (969.79 ± 6.17) μm in the enalapril folic acid group was higher with significantly(P ＜0.05).Conclusion enalapril folic acid can effectively reduce levels of peripheral blood Hey,TNF-oα,hs-CRP and OX40L in type H hypertensive rats,improve levels of NO,reduce inflammation,and improve the structure of carotid artery.
