Application of human urine 8-hydroxy deoxyguanosine detection by HPLC method in clinical tumor diagnosis
10.13699/j.cnki.1001-6821.2017.17.026
- VernacularTitle:基于HPLC法的人尿液8-羟基脱氧鸟苷检测在临床肿瘤诊断的应用
- Author:
Chen YU
1
;
Chao-Ran DONG
;
Xiao-Zheng KANG
;
Zhao-Hui ZHANG
;
Jian HUANG
;
Hui-Hui ZENG
Author Information
1. 北京大学天然药物及仿生药物国家重点实验室
- Keywords:
8-hydroxy deoxyguanosine;
tumor marker;
HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(17):1701-1705
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the detection of 8-hydroxy deoxyguanosine (8-OHdG) in urine and to explore its value in tumor-assisted diagnosis.Methods The chromatographic separation was performed on Agilent HC-C1sColumn (4.6 mm ×250 mm,5 μm)with the mobile phase of 90％ 50 mmol · L-1 NaH2PO4aqueous solution and 10％ methanol at a flow rate of 1.0 mL · rain-1 The detection wavelength was 300 nm.The injection volume was 50 μL The concentration of 8-OHdG in the urine of patients with cancer was detected by HPLC.The control group was healthy person and the patients were divided into two groups:before and after the operation,before and after chem-otherapy.Results The method had a good linear relationship.The limit of detection was 0.1 μg · mL-1.The RSDs of imer-day and intra-day precision were less than 2％.The spiked recovery rates were more than 90％.The level of 8-OHdG in lung cancer group was (5.49 ± 5.48)μg · mL-1,the level of 8-OHdG in lung cancer group was (0.51 ± 0.63) μg · mL-1,the level of 8-OHdG in healthy group was (1.16 ± 1.65) μg · mL-1.The concentration of 8-OHdG in preopretive group was significantly higher than that in postoperative group and control group (all P ＜ 0.05).Conclusion 8-OHdG detected by HPLC has high accuracy and specificity in tumor diagnosis and evaluation of chemotherapy efficacy.
