Determination the cotents of oleanolic acid in Hemsleya sinesis Cogn.from different localities by HPLC-evaporative light scattering detector
10.13699/j.cnki.1001-6821.2017.16.019
- VernacularTitle:高效液相色谱-蒸发光散射法测定不同产地雪胆中齐墩果酸的含量
- Author:
Xiao-Dong QIN
1
;
Xian-Qin LUO
;
Qing LI
;
Ting DENG
;
Rong HU
Author Information
1. 重庆市中医院药剂科
- Keywords:
Hemsleya sinesis Cogn.;
oleanolic acid;
high performance liquid chromatography-evaporative light scattering detector
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(16):1582-1584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for the determiantion of oleanolic acid in Hemsleya sinesis Cogn.from different localities.Methods The analysis was carried out with Kromasil C18(4.6 mm ×250 mm,5 pm) colunm at a column temperature of 35 ℃ and the mobile phase consisted of methanol-water-acetic acid (270:30:0.2) at a flow rate of 0.6 mL · min-1.The drift tube tempera ture for the ELSD was set at 85 ℃,and the flow rate of air was 2.70L · min-1 Results The calibration curves were linear within the range of 0.11-1.70 μg(y =23.11x + 14.90,r =0.999 9).The average recovery was 99.18％,RSD =0.78％.The highest content of Wuyanling produced in Zhejiang is up to (0.18 ± 0.05)％.The lowest content produced in Guangxi Longzhou is (0.08 ± 0.02)％.Conclusion There were some differences in the contents of oleanolic acid in Hemsleya sinesis Cogn.from different localities because of environmental factors.
