Effect of microRNA-23a on proliferation and invasion in breast cancer cells
10.13699/j.cnki.1001-6821.2017.16.015
- VernacularTitle:微小RNA-23a对乳腺癌细胞增殖和侵袭的影响
- Author:
Ke JIN
1
;
Jian-Mei ZHU
;
Fa-Qi QIU
Author Information
1. 四川省医学科学院、四川省人民医院城东病区肿瘤科
- Keywords:
breast cancer;
microRNA-23a;
proliferation;
invasion
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(16):1568-1571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-23a (miR-23a) on proliferation and invasion of breast cancer cells.Methods Recombinant human breast cancer MCF-7 and MDA-MB-435S were inoculated into 6-well plates and randomly divided into six groups,RNAi Ⅰ group,over expression-Ⅰ group,control Ⅰ group,RNAi Ⅱ group,over expression Ⅱ group,control Ⅱ group.Construct miR-23a recombinant interference and over expression vector.That were transfected into MCF-7 and MDA-MB-435S cell lines with lipofectamine 2000.Results OD492 value of MCF-7 cells and MDA-MB-435S cells after 72 h culture:in over expression group could reach 0.52 ± 0.03 and 0.44 ± 0.03 respectively while they in RNAi group were only 0.36 ±0.02 and 0.31 ± 0.02.Compared with control Ⅰ group and control Ⅱ group,RNAi-Ⅰ group and RNAi-Ⅱ group had significantly different (all P ＜ 0.05).The doubling time in over expression-Ⅰ and Ⅱ groups were (26.61 ± 5.23),(29.23 ± 5.51) h,respectively,while those in RNAi-Ⅰ and RNAi-Ⅱ groups were (42.24 ± 3.47),(58.17 ±5.34) h.The proportion of M phase cells in the over expression Ⅰ and Ⅱ groups were 52.36％,51.59％,respectively,while those in RNAi-Ⅰ and RNAi-Ⅱ groups were 76.70％,74.17％.Compared with control Ⅰ group,the doubling time and proportion of M phase cells in RNAi-Ⅰ group and over expression-Ⅰ group with significantly (all P ＜ 0.05).Compared with control Ⅱ group,the doubling time and proportion of M phase cells in RNAi-Ⅱ group andover expression-Ⅱ group with significantly (all P ＜ 0.05).Conclusion The expression levels of miR-23a showed a positive correlation with proliferation and invasion of breast cancer cells.
