Effects of microRNA-19b and microRNA-185 on proliferation and apoptosis of laryngeal squamous cell carcinoma
10.13699/j.cnki.1001-6821.2017.16.014
- VernacularTitle:微小RNA-19b和微小RNA-185抑制喉鳞癌细胞的增殖与侵袭作用研究
- Author:
Kai LI
1
;
Han BAO
;
Pan-Ying ZHANG
;
Li CHEN
Author Information
1. 西安市第九医院耳鼻喉科
- Keywords:
laryngeal squamous cell carcinoma;
cell proliferation;
cell invasion;
Hep-2 cell;
cell culture
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(16):1564-1567
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the functions of microRNA(miR)-19b and miR-185 on proliferation and apoptosis of laryngeal carcinoma cells.Methods The laryngeal squamous carcinoma cell line (Hep-2 cell) as control group,construction the miR-19b and miR-185 deletion mutants by the technology of Crisp-Case 9 as miR-19b and miR-185 deletion groups,and building miR-19b and miR-185 overexpression mutants by cell transfer dyeing technology as miR-19b and miR-185 overexpression groups.Flow cytometry was used to detect the apoptosis of control group,miR-19b,miR-185 deletion groups and miR-19b,miR-185 overexpressing groups.The apoptosis of laryngeal carcinoma cells in five groups was detected by flow cytometry.The proliferation of Hep-2 cell lines (expressed by OD450nm) and the invasive ability (expressed as the number of invasive cells) were measured under different conditions.Results After 64 h culture,the proliferation ability of miR-19b and miR-185 overexpression groups and miR-19b,miR-185 deletion groups were 0.65 ± 0.01,0.63 ± 0.01,1.69 ± 0.03,1.73 ± 0.02,had significant difference with that in control group,which was 1.28 ±0.02 (P ＜0.01).The apoptosis of miR-19b and miR-185 overexpression groups and miR-19b,miR-185 deletion groups were (12.32 ± 1.39)％,(11.74 ± 1.08) ％,(3.28 ± 0.11) ％,(3.48 ± 0.12) ％,had significant difference with that in control group,which was (5.34 ± 1.27) ％ (P ＜0.01).The number of cells in over expression of miR-19b and miR-185 overexpression groups and miR-19b,miR-185 deletion groups were 12 ±2,9 ± 3,47 ± 7,49 ± 8,had significant difference with that in control group,which was 36 ± 5 (P ＜ 0.05).Conclusion miR-185 and miR-19b can significantly inhibit the proliferation and invasion of laryngeal squamous cell carcinoma.
