Protective effects of ginsenosides on the levels of tumor necrosis factor-α, interleukin-6 and interleukin-10 in rats with acute lung injury
10.13699/j.cnki.1001-6821.2017.16.011
- VernacularTitle:人参皂苷预处理对急性肺损伤大鼠肿瘤坏死因子-α和白细胞介素-6及白细胞介素-10水平的影响
- Author:
Yu-Hong JI
1
;
Sen-Sen HUANG
;
Xiu-Li GONG
;
Yi-Min MAO
Author Information
1. 洛阳东方医院呼吸内科
- Keywords:
acute lung injury;
ginsenoside;
tumor necrosis factor-α;
interleukin-6;
interleukin-10
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(16):1552-1555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effects of ginsenoside on endotoxin of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats.Methods Adult rats were divided into 4 groups (10 rats in each group):blank group(0.9％ NaCl),model group,ginsenoside group (100 mg · kg-1)and control group(methylprednisolone 40 mg· kg-1).Intraperitoneal injection of LPS (5 mg · kg-1) to prepare the ALI model of rats.The drugs given at the time of 3 d and 1 d respectively before estabhshed model in ginsenoside group and control group.The oxygenation index (PO2/FiO2),and protein contem in bronchoalveolar lavage fluid (BALF) were detected.The levels of tumor necrosis factor α(TNF-α),intedeukin 6(IL-6) and interleukin 10 (IL-10)in BALF were detected by ELLISA.Results Pa02/Fi02:Compared with the blank group (485.49 ± 20.71) mmHg,model group was (269.29 ± 13.61) mmHg with statistically significant (P ＜0.01).Compared with the model group,ginsenoside group and control group were (379.74 ± 15.06),(398.08 ± 16.89) mmHg with statistically significant (all P ＜ 0.01).Protein content of BALF:Compared with the blank group (39.00 ± 5.00) μg · mL-1,model group was (3.69 ± 3.86) μg · mL-1 with statistically significam (P＜0.01).Compared with the model group,ginsenoside group and control group were (77.00 ± 6.00),(97.00 ± 11.00) μg · mL-1 with statistically significant (all P ＜ 0.01).Levels of TNF-α,IL-6 and IL-10 of BALF:Compared with the blank group (218.85 ±7.36),(10.84 ± 1.66),(50.00 ±7.21) ng · L-1,model group were (505.78 ±7.60),(61.55 ±4.08),(94.76 ±3.58) ng · L-1 with statistically significant (all P ＜0.01).Compared with the model group,ginsenoside group and control group were (346.42 ± 11.24),(338.14 ± 5.37)ng · L-1;(42.49 ± 1.26),(40.07 ±2.60)ng · L-1;(114.22 ±2.58),(119.07 ±4.08)ng · L-1 respectively with statistically significant (all P ＜ 0.01).Conclusion Ginsenoside may improve the ALI rats by up regulating the expression of anti-inflammatory factors (IL-10) and down regulating the expression of inflammatory factors (TNF-α and IL-6).
