Influence of sorafenib on the proliferation of cervical cancer HeLa cells and its mechanism of tumor suppression
10.13699/j.cnki.1001-6821.2017.15.024
- VernacularTitle:索拉非尼对宫颈癌HeLa细胞增殖的影响及其抑癌机制探讨
- Author:
Ju-Hua TANG
1
;
Han WANG
Author Information
1. 三亚市人民医院妇产科
- Keywords:
sorafenib;
cervical cancer;
HeLa cell;
vascular endothelial growth factor-C;
phosphorylated extracellular signal-regulated kinase
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(15):1460-1463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of sorafenib on the proliferation of cervical cancer HeLa cells and explore the mechanism of its antitumor mechanism.Methods Cervical cancer HeLa cells were treated with sorafenib at a final concentration of 0.01,0.1,1.0,2.5,5.0 mg · L-1 (experimental group).Control group was not given any drug treatment (HeLa cells).The proliferation of cervical cancer HeLa cells cultured for 1,2,3 d were detected by MTT assay.The expression of vascular endothelial growth factor-C (VEGF-C) and phosphorylated extracellular signal-regulated kinase (p-ERK) protein were detected by immunofluorescence assay and enzyme-linked immunosorbent assay (ELISA).Results After 3 d,the proliferation inhibition rates in 0.01,0.1,1.0,2.5,5.0 mg · L-1 experimental groups were (9.16 ± 1.58)％,(23.07 ± 1.86)％,(30.86 ± 2.67)％,(55.94 ± 3.92)％,(68.75 ±4.48)％,had significant difference with that in control group,which was (5.15 ± 1.67)％ (P ＜0.05).Immunofluorescence assay showed that,the green fluorescence in control group enhanced significantly,and the green fluorescence decreased significantly after treated with different concentrations of sorafenib.The expression of VEGF-Cin 0.01,0.1,1.0 mg L-1 experimental groups were (1305.64 ± 119.82),(1235.57 ± 102.34),(1083.33 ±89.61)pg · mL-1,had significant difference with that in control group,which was(1326.54 ± 139.57)pg· mL-1(P ＜0.05).The expression of p-ERK in 0.01,0.1,1.0 mg · L-1 experimental groups were (2.21 ±0.22),(1.56 ± 0.21),(0.96 ± 0.13) μg · mL-1,had significant difference with that in control group,which was(2.34 ± 0.28)μg · mL-1 (P ＜ 0.05).The expression of VEGF-C and p-ERK protein in HeLa cell culture supernatant decreased gradually with the increasing the sorafenib concentration (P ＜ 0.05).Conclusion Sorafenib can inhibit the proliferation of cervical cancer HeLa cells,and the proliferation inhibition rate is positively related to time and concentration.The mechanism of tumor suppressor may be related to its ability to inhibit the expression of VEGF-C and p-ERK protein in cervical cancer HeLa cells.
