Negative regulation of MiR-101 on the proliferation of colon cancer HCT116 cells and its mechanism
10.13699/j.cnki.1001-6821.2017.15.022
- VernacularTitle:MiR-101负性调节人结肠癌细胞HCT116增殖机制的研究
- Author:
Yuan-Yuan WANG
1
;
Xia JIANG
;
Xing-Chun XIAO
;
Chun-Lin ZHAO
;
Qiang ZHANG
;
Ying-Chao GAO
;
Lin-Lin CHEN
;
Ying-Yu MA
;
Zeng-Ren ZHAO
Author Information
1. 河北医科大学附属第一医院普外科
- Keywords:
colon cancer;
microRNA;
MiR-101;
Notch 1;
cell proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(15):1454-1456,1468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects and the molecular mechanism of miR-101 on the proliferation of colon cancer HCT116 cells.Methods The expression of miR-101 was regulated and treated by negative control (control group),inhibitor-miR-101 (experimental Ⅰ group),mimic-miR-101 (experimental Ⅱ group)for48 h,and the cell proli-feration was tested by [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazo-lium,MTS] assay.The Notch1 expression was also measured by Western blotting.Results The expression of miR-101 in experimental Ⅰ group,experimental Ⅱ group and control group were 0.467,1.767,0.967,respectively.The expression of miR-101 was significantly over-expressed by mimic-miR-101,or significantly inhibited by inhibitormiR-101,compared with control group,the differences were statistica-lly significant (all P ＜ 0.05).After 72 h treatment,the OD values of experimental Ⅰ group,experimental Ⅱ group and control group were 1.10,0.76,0.91.After 96 h treatment,the OD values of experimental Ⅰ group,experimental Ⅱ group and control group were 1.57,0.92,1.20.Compared with control group,the cell proliferation in experimental Ⅱ group were significantly inhibited,the differences were statistically significant (all P ＜ 0.05).The negatively regulated effect of miR-101 on the proliferation of HCT116 cell was taken by targeting Nocth1 expression.Conclusion As a cancer suppressor gene,miR-101 negatively regulated the proliferation of colon cancer HCT116 cells by targeting noth1 expression.
