Effects of neuropeptide Y on GluR2 subunit in hippocampal AMPA receptor in epileptic rats
10.13699/j.cnki.1001-6821.2017.15.020
- VernacularTitle:神经肽Y对癫痫大鼠海马AMPA受体GluR2亚单位功能的影响
- Author:
Hui-Ling REN
1
;
Wei BU
;
Qing-Lin ZHAO
;
Da-Wei ZHAO
Author Information
1. 河北医科大学第三医院神经内科
- Keywords:
neuropeptide Y;
epilepsy;
GluR2
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(15):1447-1449,1453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of neuropeptide Y (NPY) on GluR2 subunit in hippocampal AMPA receptor with epileptic rats by injecting kainic acid (KA).Methods The adult male SD rats with epileptic model were builded by KA.The rats were randomly divided into control,model,test A and test B groups.Control group was given 0.9％ NaCl 1 μL,intraventricular injection.Model group was given 5.5 μg · μL-1 KA 1 μL,intraventricular injection.Test A group was given 5 μg · μL-1 NPY 1 μL,intraventricular injection.Test B group was given 3 μg · μL-1 BIBP3226 1 μL,then given 5 μg · μL-1 NPY 1 μL after half hour,intraventricular injection.After 24 h,the test A and B groups received 5.5 μg · μL-1 KA 1 μL,intraventricular injection.The total protein and phosphorylation protein of GluR2 in hippocampal CA3 were measured by Western blot,and the expression of GluR2 mRNAin hippocampal CA3 were measured by real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results The total protein of GluR2 in control,model,test A and test B groups were 0.63 ± 0.21,0.63 ± 0.36,0.63 ±0.28 and 0.61 ±0.22 without significant difference (all P ＞ 0.05).In control,model,test A and test B groups,the phosphorylation protein of GluR2 were 0.42 ± 0.21,1.24 ± 0.17,0.91 ± 0.07 and 1.05 ± 0.08,the GluR2 mRNA were 1.01 ± 0.14,0.42 ± 0.16,0.76 ± 0.07 and 0.60 ± 0.09.There were statistically significant differences between control group and other three groups (all P ＜ 0.05),and also between test A group and model,test B groups (all P ＜ 0.05).Conclusion NPY could inhibit the epileptic seizure of rats,which can reduce the AMPA receptor GluR2 subunit function were inhibited by epilepsy,with activating the Y1 receptor.
