Influence of total Hedysaripolysaccharide on the level of cytokine and anti-oxidative function in acute lung injury of mice
10.13699/j.cnki.1001-6821.2017.15.019
- VernacularTitle:红芪总多糖对急性肺损伤小鼠细胞因子及抗氧化功能的影响
- Author:
Guang-Qin GENG
1
;
Xiao-Rong XIE
;
Ya-Li WANG
;
Jing SHAO
;
Hong XIANG
Author Information
1. 甘肃中医药大学药学院
- Keywords:
total Hedysaripolysaccharide;
anti-inflammatory;
antioxidant;
DNA damage
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(15):1443-1446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection of total Hedysaripolysaccharide in lipopolysaccharide (LPS) induced acute lung injury (ALI) of mice.Methods The mice were randomly divided into 6 groups:normal group,model group (8 mg · kg-1 lipopolysaccharide),control group (dexamethasone 3 mg· kg-1),and experimental groups in low,middle and high dose (50,100,200 mg · kg-1 total Hedysaripolysaccharide).Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10)levels in serum were measured with enzyme-linked immunosorbent assay.Total antioxidant capacity (T-AOC),the activities of super oxide dismutase (SOD) and malondialdehyde (MDA) contents in serum were detected by spectrophotometer.And DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis.Results The serum TNF-α and IL-10 levels in model group were (156.23 ± 7.39),(44.24 ± 2.89) ng · L-1,which in middle and high dose of experimental groups were (139.45 ± 6.57),(35.30 ± 1.88);(120.13 ± 6.81),(33.11 ±2.71) ng · L-1,the difference was significant(all P ＜0.01).T-AOC,SOD activities in middle and high dose of experimental groups were (5.91 ± 0.10),(70.00 ± 3.04);(6.10 ± 0.09),(76.21 ± 4.03) U · mg-1,which were obviously higher than those of model group which were (5.56 ± 0.13),(67.68 ± 4.86) U · mg-1 (all P ＜0.01).MDA contents of middle and high dose experimental groups were (5.01 ± 0.11),(4.17 ± 0.12) nmol · mg-1,which were obviously lower than that of model group with(5.55 ±0.11) nmol · mg-1 (all P ＜0.01).DNA tail length (TL) were (5.81 ±0.10),(5.24 ±0.10) μm,tail moment (TM)were (2.86 ±0.12),(2.71 ±0.16) μm,and Olive tail moment(OTM) were (2.67 ±0.10),(2.17 ±0.18) μm in middle and high dose of experimental groups,which were significantly lower than those of model group:TL was (6.45 ± 0.08) μm,TM was (3.22 ± 0.14) μm,and OTM was (3.35 ± 0.12) μm,the differences were all significant (all P ＜ 0.01).Conclusion Total Hedysaripolysaccharide had a protective effect against the lipopolysaccharide-induced acute lung injury.The possible mechanisms may be due to the regulation the proportional imbalance of TNF-α and IL-10,as well as the balance oxidative and anti-oxidative.
