Effects of cold on the function of colon in rats
10.13699/j.cnki.1001-6821.2017.14.015
- VernacularTitle:寒冷刺激对大鼠的结肠功能障碍的影响
- Author:
Ting-Hui NIU
1
;
Li-Dong DU
;
Guo-Tai WU
;
Zhen-He CHEN
;
Yuan REN
Author Information
1. 甘肃中医药大学药学院
- Keywords:
cold 0.9％ sodium chloride;
colon;
motor dysfunction;
defecation dysfunction
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(14):1340-1343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of cold on the function of co lon in Sprague Dawley (SD) rats.Methods Eighty SD rats were randomly divided into normal group and experimental group.The rats in experimental group were orally administrated 0-4 ℃ cold saline for 60 days.The intake and drinking in 24 hours,the faeces quantity within 6 hours and the time of defecation were measured on 30 and 60 days.Blood biochemical parameters were measured with an automatic biochemical analyzer.Faeces were collected and observed by stereo microscope.A part of colonand gastric was fixed with 10％ for malin.Hematoxylin-eosin stained,and then observed under microscope.Results On 60 days,the weight of normal group and experimental group were (268.2 ± 18.35),(319.2 ± 10.17) g,and the intake in 24 hours were (48.0 ± 1.7),(85.1 ± 2.6) mg · g-1 and drinking in 24 hours were (54.6 ± 9.5),(84.6 ± 2.7) μL · g-1,the faeces quantity within 6 hours were (0.65±0.43),(3.06 ± 0.62) g,the time of defecation were (615.5±156.48),(408.2 ± 29.7) min.The difference between two groups above factors had statistical significance (all P ＜ 0.01).On 60 days,the serum Albumin (Alb) in experimental group was (38.48 ± 2.95) g · L-1,and the creatinine (Cr) was (36.46 ± 2.07) mmol · L-1,and the blood urea nitrogen (BUN) was (6.38 ± 0.46) mmol · L-1,and the concentration of sodium (Na +) was (142.04 ±4.43) mmol · L-1,and the concentration of potassium (K+) was (5.98 ± 1.03) mmol · L-1.There was no significant difference between two groups (all P ＞ 0.05).Observation of the morphology of colon that the mucosa epithelial cells and goblet cells in experimental group were increased,and the neutrophil were reduced and a small amount of inflammatory cell are infiltrating after 60 days.The rat feces become into beaded,hard and unpolished after 60 days.Conclusion Cold stimulation could lead to colonic motility and secretion dysfunction in SD rat.
