Effect of emodin on the levels of hypoxia inducible factor-1 α and glycogen synthase kinase-3β in rats with severe acute pancreatitis
10.13699/j.cnki.1001-6821.2017.14.013
- VernacularTitle:大黄素对重症胰腺炎肾损伤模型大鼠血清缺氧诱导因子-1α及糖原合酶激酶-3β水平的影响
- Author:
Wen-Shuai LIU
1
Author Information
1. 德州市人民医院重症监护室
- Keywords:
emodin;
severe acute pancreatitis renal injury;
serum hypoxia inducible factor-1α;
glycogen synthase kinase-3β
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(14):1332-1335
- CountryChina
- Language:Chinese
-
Abstract:
Alstract:Objective To study the effect of emodin on the levels of hypoxia inducible factor-1 α (HIF-1 α) and glycogen synthase kinase-3β (GSK-3β) in rats with severe acute pancreatitis (SAP).Methods A total of 60 rats were randomly divided into blank group,sham-operation group,model group,control group and test group,each group had 12 rats.Model and test groups were injected with sodium taurocholate 0.1 mL / 100 g to establish rat model of severe pancreatitis with renal injury.Sham-operation and control groups were gently flipped the duodenum and pancreas after opening the abdomen.Blank group was not given any treatment.Blank,sham-operation and model groups were given 5 μL · g-1 0.9％ NaCl q6 h,intraperitoneal injection.Control and test groups were given 5 g · L-1 emodin with the dose was 25 μg · g-1 q6 h,intraperitoneal injection.The serum creatinine (SCr),urea nitrogen (BUN),HIF-lαt and GSK-3β were compared between the five groups after operation 3,6 and 12 h.Results The pancreatic histopathology score in model group was (9.84 ± 1.05) points,which in blank,sham-operation,control and test groups were (1.30 ± 0.14),(1.39 ± 0.14),(1.40 ± 0.15),(2.73 ± 0.30) points,the differences were significant (P ＜ 0.05),and there were significant difference between test group and blank,sham-operation,control groups (P ＜ 0.05).After operation 3 h,the main indexes in blank,sham-operation,control,model,test groups were compared,SCr were (60.46 ± 6.78),(61.52 ± 6.78),(60.38 ± 6.74),(120.57 ± 13.43),(95.35 ± 9.85) μmol· L-1;BUN were(7.47 ±0.79),(8.02 ±0.92),(7.93 ±0.83),(12.49 ± 1.53),(8.56 ±0.89) mmol· L-1;HIF-1α were (225.46 ±23.57),(210.57 ±23.58),(229.67 ±6.74),(160.46 ±17.47),(144.57±14.85)μg· L-1;GSK-3β were (6.59±0.69),(6.57 ±0.69),(6.74±0.68),(19.95 ±2.13),(10.56 ± 1.39) μg · L-1.After operation 6 h,the main indexes in blank,sham-operation,control,model,test groups were compared,SCr were (60.57 ±6.79),(60.55 ±6.76),(59.50 ±6.76),(143.57 ± 15.47),(110.57 ±12.55)μmol· L-1;BUN were (7.65 ±0.84),(8.11 ±0.93),(8.29±0.92),(15.46±1.64),(12.35 ± 1.37)mmol · L-1;HIF-1α were (226.46 ±24.04),(222.46 ±23.57),(230.57 ±24.05),(155.36 ± 15.74) (127.57 ± 12.84) μg · L-1GSK-3β were (6.25 ±0.67),(6.71 ±0.69),(6.82 ±0.71),(25.34 ±2.64),(11.56 ± 1.27)μg · L-1.After operation 12 h,the main indexes in blank,sham-operation,control,model,test groups were compared,SCr were (60.61 ± 6.79),(61.68 ± 6.79),(61.54 ± 6.78),(166.45 ±17.05),(131.45 ±13.46) μmol · L-1;BUN were (8.03 ±0.85),(7.98 ±0.82),(8.79 ±0.93),(18.66±19.46) (15.35 ± 1.63)mmol · L-1-,HIF-1α were (219.57 ±23.63),(226.35 ±24.04),(220.25 ±24.04) (148.46 ± 18.94) (117.46 ± 12.04) μg · L-1,,GSK-3β were (6.77 ± 0.69),(6.70 ±0.70),(6.86 ±0.70),(35.02 ± 3.76),(20.35 ± 2.13) μg · L-1.After operation 3,6 and 12 h,the SCr,BUN,HIF-1 α and GSK-3β in blank,sham-operation and control groups were significantly different with those of model group (P ＜ 0.05),and those indexes were significantly different between test and model groups after operation 3,6 and 12 h (P ＜ 0.05).Conclusion Emodin may enhance the ability of renal cells to hypoxia by increasing the level of serum HIF-1α and GSK-3β,thus playing a protective role in rats of severe acute pancreatitis with renal injury.
