Effect of toll-like receptor 4 / nuclear factor-kappa B pathway and its related inflammatory markers on peripheral facial neuritis
10.13699/j.cnki.1001-6821.2017.14.009
- VernacularTitle:Toll样受体4/核转录因子kappa B信号通路及其相关炎症因子对周围性面神经炎的影响
- Author:
Jian-Hui YAN
1
;
Li-Juan HUANG
;
Jian-Ping LIU
;
Hui OUYANG
;
Gao-Mei LUO
;
Wen-Ting WANG
;
Yan-Bing CHEN
Author Information
1. 湘南学院附属医院老年综合内科
- Keywords:
peripheral facial neuritis;
peripheral blood mononuclear cells;
toll-like receptor 4 / nuclear factor-kappa B pathway;
inflammatory factor
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(14):1317-1320
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of toll-like receptor 4 (TLR 4) / nuclear factor-kappa B (NF-κB) pathway and its related inflammatory markers on peripheral facial neuritis.Methods Fifty patients with peripheral facial neuritis were enrolled in the treatment group,and fifty healthy subjects were enrolled in the control group.Real-time fluorescent quantitative PCR (RT-qPCR) and Western blot was used to detect the expression of TLR4 mRNA and protein.Western blot were used to detect NF-κB p65 phosphorylation in peripheral blood mononuclear cell (PBMCs).Enzyme linked immunosorbent assay (ELISA) was used to detect the content of serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β).Results The relative expression levels of TLR4 mRNA in PBMCs of treatment group and control group were 1.55 ± 0.25 and 0.83 ±0.10,while that of TLR4 protein were 1.11 ±0.17,0.61 ±0.11;the phosphorylation levels of NF-κB p65 in treatment group and control group were 0.67 ± 0.30,0.36 ±± 0.22;the serum TNF-α in the treatment group and the control group were (149.50±12.12),(102.10±10.60) ng L-1,while the serum IL-1β3 were (255.50 ± 19.19),(105.20 ± 12.80)ng · L-1,the differences were statistically significant (P ＜0.05).Conclusion The expression changes of TLR4/NF-κB pathway in peripheral blood mononuclear cells and related in flammatory markers in serum may play a role in peripheral facial neuritis.
