Expression of Exogenous Human Hepatic Nuclear Factor-1alpha by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2.
10.3347/kjp.2011.49.4.431
- Author:
Shunyao LIAO
1
;
Yunqiang LIU
;
Bing ZHENG
;
Pyo Yun CHO
;
Hyun Ok SONG
;
Yun Seok LEE
;
Suk Yul JUNG
;
Hyun PARK
Author Information
1. Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Iksan 570-749, Korea. hyunpk@wonkwang.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Plasmodium falciparum;
hepatic nuclear factor-1alpha;
subtilisin-like protease 2;
lentiviral vector
- MeSH:
5' Untranslated Regions/*genetics;
Animals;
Cell Line;
DNA, Protozoan/genetics;
Gene Expression Regulation/*genetics;
Genetic Vectors;
Hepatocyte Nuclear Factor 1-alpha/administration & dosage/genetics/*metabolism;
Host-Parasite Interactions;
Humans;
Injections, Intravenous;
Lentivirus/genetics;
Malaria, Falciparum/metabolism/*parasitology/pathology;
Mice;
Plasmodium falciparum/drug effects/*genetics;
Promoter Regions, Genetic/genetics;
RNA, Messenger/genetics;
RNA, Protozoan/genetics;
Recombinant Proteins;
Signal Transduction;
Subtilisins/*genetics/metabolism
- From:The Korean Journal of Parasitology
2011;49(4):431-436
- CountryRepublic of Korea
- Language:English
-
Abstract:
The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1alpha is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1alpha in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1alpha expressed by a lentiviral vector (LV HNF-1alpha) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1alpha was observed to influence promoter activity, suggesting that host HNF-1alpha interacts with the Sub2 gene.
