Effect of lobaplatin on radiosensitivity of human cervical carcinoma SiHa cells
10.13699/j.cnki.1001-6821.2017.13.017
- VernacularTitle:洛铂对宫颈癌SiHa细胞射线敏感性的影响
- Author:
Ze WANG
1
;
Guo-Jun WANG
Author Information
1. 西南医科大学药学院
- Keywords:
Lobaplatin;
radisosensitivity;
cervical carcinoma;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(13):1229-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lobaplatin on the radiosensitivity of cervical cancer SiHa cells and its mechanism.Methods MTT assay was used to detect the proliferation of SiHa cells treated with different concentrations of lobaplatin at 24,48 and 72 h,and the IC50 values were calculated.The radiosensitivity of SiHa cells were measured by colony formation assay,and the multi-target single hit model was used to fit the radiological parameters of SiHa cells.Flow cytometry was used to detect the effect of lobaplatin,radiation and lobaplatin combined with radiation on the apoptotic rate of SiHa cells.Western blot was used to detect the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2 in SiHa cells.Results According to the results of MTT assay and colony formation assay,cells were divided into four groups as follows:blank group (routine cell culture),lobaplatin group (5.0 μmol · L-1 lobaplatin),radiation group (6 Gy 6 MV X-ray) and lobaplatin + radiation group(5.0 μmol · L-1 lobaplatin + 6 Gy 6 MV X -ray).The apoptosis rates of the blank group,the lobaplatin group,the radiotherapy group and the combination group were (4.44 ± 2.07) ％,(30.50 ± 3.17)％,(43.08 ± 5.93)％ and (84.68 ±4.90)％,respectively.The expression of pro-apoptotic protein Bax was significantly up-regulated and the expression of Bcl-2 was down-regulated in the lobaplatin group and the radiotherapy group compared with the blank group.Compared with the lobaplatin group and the radiotherapy group,the expression of pro-apoptotic protein Bax was significantly up-regulated while the expression of Bcl-2 was down-regulated in the combination group.Conclusion Lobaplatin might enhance the radiosensitivity of SiHa cells through the apoptosis pathway.
