Quick LC-MS/MS method for the determination of daptomycin in human plasma
10.13699/j.cnki.1001-6821.2017.12.021
- VernacularTitle:LC-MS/MS法快速测定人血浆中达托霉素浓度
- Author:
Chang-Qian XIAO
1
;
Ying-Chun XIANG
;
Qi HAN
;
Li LI
Author Information
1. 浙江医院药剂科
- Keywords:
daptomycin;
LC-MS/MS;
plasma drug concentration;
pharmacokinetics
- From:
The Chinese Journal of Clinical Pharmacology
2017;33(12):1149-1151,1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a LC-MS/MS method for determination of daptomycinin human plasma.Methods The plasma samples were precipitated by acetonitrile,then the supernatant were separated on Eclipse Plus C18column (2.1 mm × 100 mm,3.5 μm) and eluted with acetonitrile-10 mmol · L-1 ammonium acetate (80∶ 20,containing 0.1％ formic acid),and the column temperature was 25 ℃.Detection of the analyte was achieved using positive ion electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode.The MS/MS ion transitions monitored were m/z 811.0→m/z 159.1 and m/z 237.1→m/z 194.1 for daptomycin and internal standard,respectively.The specificity,standard curve and lower limitation of quantitation,precision and recovery rate and stability as well as the matrix effect were investigated.Results The linear regression equation was y =1.49 × 10-2x-2.06 × 10-3 (r =0.9980) The linear range of daptomycin was 0.10-100 μg · mL-1,with the lower limit of quantitation (LLOQ) of 0.10 μg · mL-1.Accuracy was 97.1％-104.8％,Intra-day and inter -dayrelative standard deviations were both below 15％.The absolute recovery in plasma was81.9％-91.4％.The matrix effects were 90.01％-109.28％.Conclusion The established method israpid,sensitive,accurate,specific and reliable,and suitable for the determination ofdaptomycin in human plasma and pharmacokinetic study.
